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Purified anti-Tau, 6-18 Antibody (Previously Covance catalog# SIG-39416)

Pricing & Availability
Clone
Tau 12 (See other available formats)
Regulatory Status
RUO
Other Names
PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1, DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND
Previously
Signet Catalog# 9416-02
Signet Catalog# 9416-05
Signet Catalog# 9416-10
Covance Catalog# SIG-39416
Isotype
Mouse IgG1
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Product Citations
publications
Tau_12_Purified_Tau_6-18_Antibody_1_012918
Western blot of anti-Tau, 6-18 antibody (clone Tau 12). Lane 1: Molecular weight marker; Lane 2: Tau protein ladder; Lane 3: 20 µg of human brain lysate. The blots were incubated with 0.05 µg/mL of Tau 12 or mouse IgG1 overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • Tau_12_Purified_Tau_6-18_Antibody_1_012918
    Western blot of anti-Tau, 6-18 antibody (clone Tau 12). Lane 1: Molecular weight marker; Lane 2: Tau protein ladder; Lane 3: 20 µg of human brain lysate. The blots were incubated with 0.05 µg/mL of Tau 12 or mouse IgG1 overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • Tau_12_Purified_Tau_6-18_Antibody_2_012918
    IHC staining of anti-Tau, 6-18 antibody (clone Tau 12) on formalin-fixed paraffin-embedded human Alzheimer’s disease brain tissue. The tissue was incubated with 0.05 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • Tau_12_Purified_Tau_6-18_Antibody_3_012918
    Direct ELISA of anti-Tau, 6-18 Antibody (clone Tau 12) and isotype-matched control mouse IgG1 binding to plate-immobilized recombinant human 2N4R Tau protein. ELISA was performed by coating wells with 150 ng of recombinant human 2N4R Tau protein. The wells were then incubated with Tau 12 or mouse IgG1 at 37°C for 45 minutes, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
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806502 500 µL 1648 CHF
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806503 25 µL 112 CHF
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806501 100 µL 407 CHF
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P, ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.05 µg per ml. For immunohistochemistry, a concentration range of 0.01 - 0.05 µg/ml is suggested. For ELISA applications, a concentration range of 10 - 100 ng/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is specific for an epitope that lies between amino acids 6-18 of human Tau.

Application References

(PubMed link indicates BioLegend citation)
  1. Irwin D, et al. 2012. Brain. 135(Pt 3):: 807. (IHC-P) PubMed
  2. Horowitz PM, et al. 2004. J Neurosci. 24(36):7895 (WB)
  3. Sengupta U, et al. 2017. Ann Clin Transl Neurol. 4(4):226. (WB) PubMed
  4. Meredith JE, et al. 2013. PLoS One. 7;8(10):e76523 (ELISA, WB) PubMed
  5. Song L, et al. 2015, Mol Neurodegener. 10:14 (ELISA, WB) PubMed
Product Citations
  1. Snellman A, et al. 2022. Brain. 145:2834. PubMed
  2. Montoliu-Gaya L, et al. 2023. Nat Aging. 3:661. PubMed
  3. Ashton NJ, et al. 2022. EBioMedicine. 76:103836. PubMed
  4. Chatterjee S, et al. 2022. Sci Adv. 8:eabm6063. PubMed
  5. Ercan E, et al. 2017. Mol Neurodegener. . 10.1186/s13024-017-0229-1. PubMed
  6. Haenig C, et al. 2020. Cell Rep. 32:108050. PubMed
  7. Karikari TK, et al. 2020. Lancet Neurol. 422:19. PubMed
  8. Serafín V, et al. 2020. Anal Bioanal Chem. 413:799. PubMed
  9. Milà-Alomà M, et al. 2022. Nat Med. :. PubMed
  10. Sengupta U, et al. 2017. Ann Clin Transl Neurol. 10.1002/acn3.382. PubMed
  11. Karikari TK, et al. 2020. ACS Chem Neurosci. 2.622916667. PubMed
  12. Karikari TK, et al. 2020. Alzheimers Dement. 17:755. PubMed
  13. Bichmann M, et al. 2021. Mol Neurodegener. 16:46. PubMed
RRID
AB_2564707 (BioLegend Cat. No. 806502)
AB_2564707 (BioLegend Cat. No. 806503)
AB_2564707 (BioLegend Cat. No. 806501)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: Cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with: Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, and alpha-synuclein.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References

1. Meredith JE Jr, et al. 2013. PLoS One. 8(10): e76523. PubMed
2. Goodall CA, et al. 2006. J. Neurol. Neurosurg. Psychiatry 77(1): 89. PubMed
3. Wang Y, Mandelkow E. 2016. Nat. Rev. Neurosci. 17(1):5-21. PubMed

Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau 6-18 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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