Purified anti-TCP-1β Antibody

Pricing & Availability
Clone
F39P7F11 (See other available formats)
Regulatory Status
RUO
Other Names
CCT beta, CCTB, T-complex protein 1 subunit beta, TCP1 beta
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_F39P7F11_Pure_TCP-1B_Antibody_113018
Total cell lysates (15 µg total protein) from HeLa, A549, HEL and Raw264.7 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TCP-1β Antibody, clone F39P7F11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1_F39P7F11_Pure_TCP-1B_Antibody_113018
    Total cell lysates (15 µg total protein) from HeLa, A549, HEL and Raw264.7 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-TCP-1β Antibody, clone F39P7F11, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 2_TCP-1beta_F39P7F11_111010
    HepG2 cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal TCP-1β antibody (clone F39P7F11). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
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635502 100 µg 229 CHF
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Description

TCP-1β (Chaperonin containing T complex polypeptide 1, subunit 2) belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD. Chaperonin-containing TCP-1 is a large multisubunit complex of 800-900 kD, a protein complex considered to be the cytosolic homologue of mitochondrial HSP60. TCP-1β has been shown to interact with β-actin, 14-3-3, SUMO4, TCP-1α, β-Tubulin, α-Tubulin and CCT8. In vitro, TCP-1βmediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.Clone F39P7F11has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat and Frog TCP-1β.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Frog
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ovalbumin-conjugated synthetic Peptide RKRVPDHHPC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 µg per 5 ml antibody dilution buffer for each mini-gel.  It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Coghlin C, et al. 2006. J Pathol. 210:351. PubMed
Product Citations
  1. Coghlin C, et al. 2006. J Pathol. 210:351. PubMed
  2. Arines FM, et al. 2021. J Cell Biol. 220:. PubMed
RRID
AB_2275598 (BioLegend Cat. No. 635502)

Antigen Details

Structure
Belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD.
Distribution

Cytoplasm

Function
In vitro, TCP-1β mediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.
Interaction
β-actin, 14-3-3γ, SUMO4, TCP-1α, β-Tubulin, α-tubulin, CCT8
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure
Antigen References

1. Yaffe MB, et al. 1992. Nature 358:245

Gene ID
21454 View all products for this Gene ID
UniProt
View information about TCP-1 beta on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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