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Purified anti-TDRD3 Antibody

Pricing & Availability
Clone
W16174A (See other available formats)
Regulatory Status
RUO
Other Names
Tudor domain-containing protein 3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1_W16174A_Pure_TDRD3_1_WB_HR_Antibody_081517
Total cell lysate (15 µg protein) from U87-MG, HeLa and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 (2 µg/mL) diluted purified anti-TDRD3 antibody (clone W16174A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (clone Poly4054) for clone W16174A or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP (clone Poly4054) for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder.
  • 1_W16174A_Pure_TDRD3_1_WB_HR_Antibody_081517
    Total cell lysate (15 µg protein) from U87-MG, HeLa and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 (2 µg/mL) diluted purified anti-TDRD3 antibody (clone W16174A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (clone Poly4054) for clone W16174A or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP (clone Poly4054) for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder.
  • 2_W16174A_Pure_TDRD3_2_IF_HR_Antibody_081417
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with anti-TDRD3 (clone W16174A) overnight at 4 degree followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG (clone Poly4054) incubation for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 3_W16174A_Pure_TDRD3_1_IF_HR_Antibody_081417
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:250 (2 µg/mL) rat IgG2b, κ isotype control (clone RTK4530) overnight at 4 degree followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG (clone Poly4054) incubation for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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601201 25 µg 101 CHF
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601202 100 µg 253 CHF
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Description

Tudor Domain Containing 3 (TDRD3) is a protein which specially recognizes di-methylarginine containing proteins. As an effector molecular, it binds to histone methylarginine H3R17me2a and H4R3me2a marked by coactivator-associated arginine methyltransferase (CARM1/PRMT4). In addition, TDRD3 recognizes the Arg1810 me2a modification in the CTD of RNA polymerase II, therefore is associated with transcriptional activation. Recent study suggests other roles other than effector in the nucleus. In cytoplasm, TDRD3 interacts with ubiquitin-specific protease 9 X-linked (USP9X) in cytoplasmic stress granules and is suggested that TDRD3 might regulate USP9X de-ubiqutinase activity. Knockdown of TDRD3 in breast cancer cells sensitizes chemotherapeutic drug-induced apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial human TDRD3 (402-651 a.a.) recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml (1:250 - 1:2500 dilution). For immunocytochemistry, the recommended usage is 0.5 - 2.0 µg per ml (1:250 - 1:1000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody does not cross-react with mouse (in-house tested).

RRID
AB_2715902 (BioLegend Cat. No. 601201)
AB_2715902 (BioLegend Cat. No. 601202)

Antigen Details

Structure
651 amino acids with a predicted molecular weight of 73 kD. Contains a C-terminal Tudor domain responsible for recognizing symmetrically dimethylated arginine.
Distribution

Cytoplasm, nucleus.

Function
TDRD3 recognizes di-methylarginine containing proteins and functions as a transcriptional activator.
Interaction
TDRD3 interacts with FMR1, FXR1, FXR2, EWSR1, FUS, SERBP1, EEF1A1, DDX3, POLR2A, SNRPB, and SNRPN.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Goto-Ito S, et al. 2017. Sci Rep. 7:42123.
2. Narayanan N, et al. 2017. Cell Discov. 3:16048.
3. Sikorsky T, et al. 2012. Nucleic Acids Res. 40:11748.
4. Yang Y, et al. 2010. Mol Cell. 40:1016.
5. Linder B, et al. 2008. Hum Mol Genet. 17:3236.
6. Goulet I, et al. 2008. Hum Mol Genet. 17:3055.

Gene ID
81550 View all products for this Gene ID
UniProt
View information about TDRD3 on UniProt.org
Go To Top Version: 0    Revision Date: 08.14.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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