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Purified anti-TFEB Antibody

Pricing & Availability
Clone
A17106C (See other available formats)
Regulatory Status
RUO
Other Names
Transcription factor EB
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_A17106C_PURE_TFEB_Antibody_030718
Western blot of purified anti-TFEB antibody (clone A17106C). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_A17106C_PURE_TFEB_Antibody_030718
    Western blot of purified anti-TFEB antibody (clone A17106C). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 5 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2_A17106C_PURE_TFEB_Antibody_2_010419.png
    IHC staining of purified anti-TFEB antibody (clone A17106C) on formalin-fixed paraffin-embedded human renal carcinoma tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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852001 25 µg 112 CHF
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852002 100 µg 276 CHF
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Description

The autophagy-lysosomal pathway is involved in the degradation of long-lived proteins. The transcription factor EB (TFEB) plays a critical role in regulating basic cellular processes, such as lysosomal biogenesis and autophagy. The subcellular localization and activity of TFEB are regulated by mechanistic target of rapamycin (mTOR)-mediated phosphorylation. TFEB is also involved in physiological processes, and it induces the intracellular clearance of pathogenic factors in a variety of diseases, such as Parkinson's and Alzheimer's, suggesting that novel therapeutic strategies could be based on the modulation of TFEB activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2728585 (BioLegend Cat. No. 852001)
AB_2728585 (BioLegend Cat. No. 852002)

Antigen Details

Structure
TFEB is a 476 amino acid protein with a molecular weight mass of approximately 53kD.
Distribution

Tissue distribution: TFEB is expressed in numerous cell types of the central nervous system (CNS).

Cellular distribution: Cytosolic and nucleus.

Function
TFEB is a major regulator of autophagy and lysosomal biogenesis.
Interaction
TFEB interacts with the mTOR complex and its activity is thought to be regulated through the phosphorylation by mTOR.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Martini-Stoica H, et al. 2016. Trends Neurosci. 221-234.
  2. Napolitano G, Ballabio A. 2016. J. Cell Sci. 129: 2475-81.
  3. Raben N, Puertollano R. 2016. Annu. Rev. Cell Dev. Biol. 32: 255-278.
Gene ID
7942 View all products for this Gene ID
UniProt
View information about TFEB on UniProt.org
Go To Top Version: 2    Revision Date: 01.04.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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