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Purified anti-TICAM-2 (TRAM) Antibody

Pricing & Availability
Clone
9G1B30 (See other available formats)
Regulatory Status
RUO
Other Names
TIR domain-containing adapter molecule 2, TRIF-related adapter molecule (TRAM), Toll-like receptor adaptor protein 3, Toll/interleukin-1 receptor domain-containing protein
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
9G1B30_TICAM2_Purified_Antibody_WB_71816
Whole cell lysates (15 µg protein) from Daudi (negative control) and THP-1 (positive control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1.0 µg/mL) purified anti-TICAM2 antibody , clone 9G1B30 . Proteins were visualized using chemiluminescence detection by incubating with HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Purified anti-GAPDH antibody (Cat. No. 631401) was used as a loading control (lower). Lane M: MW ladder.
  • 9G1B30_TICAM2_Purified_Antibody_WB_71816
    Whole cell lysates (15 µg protein) from Daudi (negative control) and THP-1 (positive control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1.0 µg/mL) purified anti-TICAM2 antibody , clone 9G1B30 . Proteins were visualized using chemiluminescence detection by incubating with HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). Purified anti-GAPDH antibody (Cat. No. 631401) was used as a loading control (lower). Lane M: MW ladder.
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659402 100 µg 265 CHF
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Description

TICAM-2, also known as TRIF-related adaptor molecule (TRAM), is a TIR domain containing protein that was found to act as an adaptor molecule during LPS-induced innate immune response. Upon infection by Gram-negative bacteria, TLR4 specifically recognizes LPS and leads to the dimerization of the cytoplasmic TIR domains. The activated TLR4 subsequently recruits the adaptor protein complex containing both TICAM-1 and TICAM-2, resulting in the activation of IFN response factors and production of pro-inflammatory cytokines. In this signaling cascade, TICAM-2 is essential for bridging the interaction between TLR4 and TICAM-1. TICAM-2 is also involved in the regulation of the IL-18 signaling pathway, acting as a sorting adaptor that recruits MYD88 from the cytosol to the membrane region upon activation of IL-1/IL-18 receptors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human TICAM-2 recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Daudi cells were selected as a negative control for antibody testing due to the cell line’s low expression level of the mRNA encoding TICAM2 (source: Human Protein Atlas).

RRID
AB_2563252 (BioLegend Cat. No. 659402)

Antigen Details

Structure
235 amino acids encode a protein with a predicted MW of 27 kD. Contains a TIR domain responsible for mediating the physical interaction with TLR4 and TICAM-1.
Distribution

Cytoplasm, golgi apparatus, endosome, cell membrane, and endoplasmic reticulum.

Function
TICAM-2 acts as a sorting adaptor molecule that transduces TLR4 signaling to TICAM-1 and regulates MYD88-independent pathway in response to LPS stimulation.
Interaction
Interacts with TRAF6, MYD88, TLR4, IL1R1, IL1RAP, IRAK2, IRAK3, TICAM1, IRF3, and IRF7.
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Antigen References

1. Enokizono Y, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:19908.
2. Ohnishi H, et al. 2012. PLoS One. 7:e38423.
3. Wu B, et al. 2011. Dev. Comp. Immunol. 35:764.
4. Seya T, et al. 2005. Int. J. Biochem. Cell Biol. 37:524.
5. Vogel SN, et al. 2003. Mol. Interv. 3:466.
6. Oshiumi H, et al. 2003. J. Biol. Chem. 278:49751.

Gene ID
353376 View all products for this Gene ID
UniProt
View information about TICAM-2 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 11.28.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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