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Purified anti-TIF1β (KAP-1, TRIM28) Phospho (Ser473) Antibody

Pricing & Availability
Clone
11G10SC (See other available formats)
Regulatory Status
RUO
Other Names
TRIM28 pS473, KAP-1 pS473
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a.11G10SC_Purified_TIF1b_pS473_Antibody_WB_031919
Total cell lysates (15 µg total protein) from HEK293T cells and untreated (-) or treated (+) with UV radiation were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti- TIF1β (KAP-1, TRIM28) Phospho (Ser473) Antibody, clone 11G10SC, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-TIF1β (KAP-1, TRIM28) Antibody, clone 20A1 (Cat. 619302), was used as a pan TIF1β loading control at 1:50000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • a.11G10SC_Purified_TIF1b_pS473_Antibody_WB_031919
    Total cell lysates (15 µg total protein) from HEK293T cells and untreated (-) or treated (+) with UV radiation were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti- TIF1β (KAP-1, TRIM28) Phospho (Ser473) Antibody, clone 11G10SC, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-TIF1β (KAP-1, TRIM28) Antibody, clone 20A1 (Cat. 619302), was used as a pan TIF1β loading control at 1:50000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • b.11G10SC_Purified_TIF1b_Antibody_ICC_011221
    HEK293 cell extracts untreated (lane 1) and treated with UV radiation (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-TIF1β phosphorylated (Ser473) antibody (clone 11G10SC). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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654101 25 µg 118 CHF
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654102 100 µg 293 CHF
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Description

Transcriptional intermediary factors (TIFs) are a group of transcriptional coactivators and corepressors that regulate gene expression by modulating chromatin structure and assembly of transcription initiation complexes. TIF1β is a member of the TIF1 subfamily of chromatin-associated TIFs that play a key role in many developmental and physiological processes. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Modified peptide: VKRSRpSGEGEVC.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Jamal K, et al. 2022. Cancer Res Commun. 2:1244. PubMed
  2. Boonen RACM, et al. 2022. Cancer Res. 82:615. PubMed
  3. Storchova R, et al. 2021. FEBS J. 288:6035. PubMed
  4. Zu R, et al. 2021. Cell Death Dis. 12:994. PubMed
RRID
AB_2561781 (BioLegend Cat. No. 654101)
AB_2561781 (BioLegend Cat. No. 654102)

Antigen Details

Structure
An N-terminal RING-B box coiled coil (RBCC) domain most likely attributes to intermolecular structure, while two characteristic chromatin-targeting domains, a plextrin homology (PH) finger motif and bromodomain, are found in the C-terminus.
Distribution

Nucleus

Function
TIF1β acts as a transcriptional repressor by modulating histone deacetlyation, histone H3 Ly-9 methylation, and recruitment of HP1 proteins.
Interaction
TIF1β was shown to form homo-oligomers in vitro, but does not form hetero-oligomers with TIF1α or TIF1γ.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Messerschmidt DM, et al. 2012. Science 335:1499.
2. Chikuma S, et al. 2012. Nat. Immunol. 13:596.

Gene ID
21849 View all products for this Gene ID
UniProt
View information about TIF1beta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 02.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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