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Purified anti-TRAF3 Antibody

Pricing & Availability
Clone
9D7C15 (See other available formats)
Regulatory Status
RUO
Other Names
TNF receptor-associated factor 3, CAP1, CD40 receptor-associated factor 1 (CRAF1), CD40-binding protein (CD40BP), LMP1-associated protein 1 (LAP1), IIAE5
Isotype
Mouse IgG1, κ
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Product Citations
publications
9D7C15_PURE_TRAF3_Antibody_122117
Total lysates (15 µg protein) from 293T and 293T/TRAF3 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) purified anti-TRAF3 antibody, clone 9D7C15 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • 9D7C15_PURE_TRAF3_Antibody_122117
    Total lysates (15 µg protein) from 293T and 293T/TRAF3 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) purified anti-TRAF3 antibody, clone 9D7C15 (upper). Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
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680802 100 µg 229 CHF
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Description

TNFR-associated factor 3, also known as TRAF3, is a member of the TNF receptor associated factor (TRAF) protein family and was originally identified as an adaptor molecule binding to the cytoplasmic tail of CD40. The engagement of CD40 with its ligand recruits the complex, which consists of TRAF3, TRAF2, and c-IAP1/2. This results in the stabilization of NF-κB inducing kinase (NIK). NIK phosphorylates the IKKα complex and subsequently leads to the activation of the RelB/p52 complex. TRAF3 is involved in the Toll-like receptor (TLR) mediated activation of proinflammatory genes such as type I interferons and IL-10. Pathogens detected by TLR3 and TLR4 recruit the adaptor protein TRIF. In turn, this activates IRF3 via the TRAF3-TBK1-IKKe complex. TRAF3 has been shown to play important roles in the regulation of innate immune response, antibody class switching, and B cell survival.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial TRAF3 recombinant protein (1-200 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566432 (BioLegend Cat. No. 680802)

Antigen Details

Structure
568 amino acids with a predicted molecular weight of 64.5 kD. Contains a C-terminal MATH/TRAF domain responsible for the binding to receptor cytoplasmic domains.
Distribution

Cytoplasm.

Function
TRAF3 mediates the signal transduction from TNF receptors and Toll-like receptors, leading to the activation of NF-κB, MAP kinases, or IRF3. TRAF3 is involved in the regulation of anti-viral response and B-cell survival.
Interaction
TRAF3 forms a homodimer. TRAF3 also forms heterodimers with TRAF2 and TRAF5. TRAF3 also interacts with CD40, TICAM1, TRAFD1, BIRC2, BIRC3, TLR4. TRIP, and TBK1.
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Hildebrand JM, et al. 2011. Immunol. Rev. 244:55.
2. Hu H, et al. 2013. Nature 494:371.
3. Jenkinson SR, et al. 2013. Proc. Natl. Acad. Sci. USA 110:21107.
4. Lin WW, et al. 2013. Front Immunol. 4:477.
5. Yi Z, et al. 2014. Cytokine Growth Factor Rev. 25:147.
6. Yi Z, et al. 2014. Nat. Immunol. 15:866.

Gene ID
7187 View all products for this Gene ID
UniProt
View information about TRAF3 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12.21.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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