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Purified anti-TRF2 Antibody

Pricing & Availability
Clone
T2JKM3 (See other available formats)
Regulatory Status
RUO
Other Names
TERF2, Telomeric repeat-binding factor 2, TRBF2, TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein,
Isotype
Mouse IgG1, κ
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Product Citations
publications
T2JKM3_PURE_TRF2_Antibody_101716
Total protein extracts (15 µg protein) from HeLa and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-TRF2 (clone T2JKM3) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
  • T2JKM3_PURE_TRF2_Antibody_101716
    Total protein extracts (15 µg protein) from HeLa and NIH3T3 were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-TRF2 (clone T2JKM3) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control.
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692402 100 µg 253 CHF
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Description

Telomeric Repeat-binding Factor 2 (TRF2) is a component of the shelterin complex, which is a six-protein complex bound to chromosome termini. The shelterin complex is essential for protecting the integrity of natural chromosome ends. Within the shelterin complex, POT1 binds single-stranded telomeric overhang, while TRF1 and TRF2 bind duplex telomeric DNA and recruit TIN2, TPP1, POT1 and Rap1 to telomeres. Inhibition of TRF2 activates DNA repair systems specifically at telomeric loci, leading to cell cycle arrest, senescence or cell death. Over-expression of TRF2 was observed in a variety of human cancers, suggesting TRF2’s roles in tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length TRF2 protein produced in baculovirus.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

TRF2 has 6 isoforms. Clone T2JKM3 recognizes multiple isoforms based on the homology of immunogen sequence. Clone T2JKM3 does not cross-react with mouse (in-house tested).

RRID
AB_2632764 (BioLegend Cat. No. 692402)

Antigen Details

Structure
542 amino acids with a predicted molecular weight of 59.6 kD.
Distribution
Nucleus.
Function
Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes.
Interaction
Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP, NBN, SLX4/BTBD12, DCLRE1B/Apollo and TERF2IP/RAP1.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Antigen References

1. Griffith JD, et al. 1999. Cell. 97:503.
2. van Steensel B, et al. 1998. Cell. 92:401.
3. Palm W, de Lange T. 2008. Annu. Rev. Genet. 42:301.
4. Bilaud T, et al. 1997. Nat. Genet. 17:236.
5. Sfeir A, de Lange T. 2012. Science 336:593.
6. Bellon M, et al. 2006. Int. J. Cancer 119:2090.
7. Benhamou Y, et al. 2016. Oncotarget. doi: 10.18632/oncotarget.10005.

Gene ID
7014 View all products for this Gene ID
UniProt
View information about TRF2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 10.24.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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