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Purified anti-TRIM Antibody

Pricing & Availability
Clone
TRIM-4 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor-interacting molecule
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
TRIM-4_Purified_TRIM_Antibody_123118.png
Total cell lysates (15 µg total protein) from Molt-4 and U87-MG (negative control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti- TRIM Antibody, clone TRIM-4, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti- β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • TRIM-4_Purified_TRIM_Antibody_123118.png
    Total cell lysates (15 µg total protein) from Molt-4 and U87-MG (negative control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti- TRIM Antibody, clone TRIM-4, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti- β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
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627202 100 µg 293 CHF
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Description

TRIM is a 30 kD disulfide-linked protein also known as T cell receptor interacting molecule. TRIM is a type III membrane protein containing a very short extracellular domain (8 amino acids). TRIM is highly expressed in T cells with some expression also observed in NK cells. TRIM is a signaling adaptor molecule that links extracellular and intracellular signaling. The TRIM protein is phosphorylated by Src kinases and associates with the PI3K regulatory subunit α after phosphorylation. TRIM has also been shown to associate with TCRα, β, and ζ. The TRIM-4 monoclonal antibody recognizes human TRIM and has been shown to be useful for Western blotting, intracellular staining by flow cytometry, and immunofluorescence staining.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial recombinant human TRIM (amino acids 29-186)
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, ICFC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: intracellular flow cytometry1, ELISA1, and immunofluorescence microscopy2.

Clone TRIM-4 has been shown to cross-react to mouse TRIM1.

Application References

(PubMed link indicates BioLegend citation)
  1. Huynh T, et al. 2001. Scand. J. Immunol. 54:146.
  2. Kirchgessner H, et al. 2001. J. Exp. Med. 193:1269.
RRID
AB_2240982 (BioLegend Cat. No. 627202)

Antigen Details

Structure
Type III membrane protein, 30 kD disulfide-linked dimeric phosphoprotein
Distribution

T cells, lower expression levels in NK cells

Function
Links extracellular and intracellular signaling pathways in T cell activation
Interaction
PI3K regulatory subunit α, TCRα, β, and ζ.
Modification
Tyrosine phosphorylation by Src kinases
Cell Type
NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Antigen References

1. Bruyns E, et al. 1998. J. Exp. Med. 188:561.
2. Hubener C, et al. 2000. Immunogenetics 51:154.

Gene ID
50852 View all products for this Gene ID
UniProt
View information about TRIM on UniProt.org

Other Formats

View All TRIM Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-TRIM TRIM-4 WB,ICC,ICFC
PE anti-TRIM TRIM-4 ICFC
Go To Top Version: 4    Revision Date: 12.31.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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