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Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P)

Pricing & Availability
Clone
Poly18020 (See other available formats)
Regulatory Status
RUO
Other Names
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, TUBB3, tubulin beta-4 chain, class III beta-tubulin
Previously
Covance Catalog# PRB-435P
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918
IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded rat cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded rat cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_Poly18020_PURE_Tubulin_beta3_IHCP_2_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_Poly18020_PURE_Tubulin_beta3_IHCP_3_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly18020_PURE_Tubulin_beta3_WB_021915.jpg
    Western blot of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.1 µg/mL of Poly18020 or Rabbit IgG overnight at 4°C, followed by incubation with HRP-labeled anti-rabbit IgG (Cat. No. 410406). Enhanced chemiluminescence was used as the detection system.
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802001 100 µL 433 CHF
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Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against microtubules derived from rat brain.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 0.5 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunocytochemistry (ICC).

This antibody is well characterized and highly reactive to neuron specific Class III ß-Tubulin (ßIII), but does not identify ß-tubulin found in glial cells. This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Feuer R, et al. 2005. J. Neurosci. 25(9):2434-44.
  2. Mozzetti S, et al. 2005. Clin. Cancer Res. 11(1):298-305.
  3. Feuer R, et al. 2003. Am. J. Pathol. 163(4):1379-93.
  4. Zhang XM, Yang XJ. 2001. Development. 128(6):943-57.
  5. Ma M, et al. 2012. Neurobiol Dis. 56:34. (WB) PubMed
  6. Tseung G, et al. 2011. J. Virol. 85:5718. (IF) PubMed
  7. Shimada IS, et al. 2012. J Neurosci. 32:7926. (IHC) PubMed
  8. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
Product Citations
  1. Ahangari N, et al. 2021. J Neuropathol Exp Neurol. 80:944. PubMed
  2. Samarasinghe RA, et al. 2021. Nat Neurosci. 24:1488. PubMed
  3. Yates PL, et al. 2021. Cell Mol Life Sci. 78:6941. PubMed
  4. Hodgetts SI, et al. 2022. Neural Regen Res. 17:1376. PubMed
  5. Muralidharan H, et al. 2022. J Neurosci. 42:2149. PubMed
  6. Jabali A, et al. 2022. EMBO Rep. 23:e54027. PubMed
  7. Reis LTC, et al. 2022. Environ Toxicol. 37:2044. PubMed
  8. Rodriguez CM, et al. 2022. Cell Rep. 41:111505. PubMed
  9. Matsubayashi J, et al. 2023. J Neurochem. 164:29. PubMed
  10. Libanori A, et al. 2023. Adv Mater. 35:e2206933. PubMed
  11. Shimizu K, et al. 2022. Cells. 11: . PubMed
  12. Olmsted ZT, et al. 2022. STAR Protoc. 3:101898. PubMed
  13. Mogas Barcons A, et al. 2022. Materials (Basel). 16: . PubMed
  14. Yates PL, et al. 2023. Front Cell Neurosci. 16:979652. PubMed
  15. Genchi A, et al. 2023. Nat Med. 29:75. PubMed
  16. Chiu YJ, et al. 2023. Biomol Ther (Seoul). :. PubMed
  17. Willumsen N, et al. 2023. Brain Commun. 5:fcac321. PubMed
  18. Yang OJ, et al. 2023. Front Neurosci. 17:1001007. PubMed
  19. Stojkovska I, et al. 2022. Neuron. 110:436. PubMed
  20. Prieto Huarcaya S, et al. 2022. Autophagy. 18:1127. PubMed
  21. Chow A, Morshead C 2016. Stem Cell Res. 16:79-87. PubMed
  22. Son M, et al. 2016. Exp Mol Med. 48: 232. PubMed
  23. Hoshino N, et al. 2021. Journal of Comparative Neurology. 529:3633. PubMed
  24. Tseng K, et al. 2017. eNeuro. 10.1523/ENEURO.0214-17.2017. PubMed
  25. Traxler L, et al. 2022. Cell Metab. 34:1248. PubMed
  26. Asamitsu S, et al. 2023. Sci Adv. 9:eade2035. PubMed
  27. Weng ZK, et al. 2023. Biomolecules. 13:. PubMed
  28. Tian JL, et al. 2023. Cells. 12:. PubMed
  29. Drobny S, et al. 2023. Transl Neurodegener. 12:31. PubMed
  30. Eltahir MK, et al. 2022. PLoS One. 17:e0269683. PubMed
  31. Pardieck J, et al. 2021. Stem Cells Dev. 30:816. PubMed
  32. Kim S, et al. 2021. Nat Commun. 12:1807. PubMed
  33. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  34. Tian E, et al. 2016. Cell Rep. 16: 781-792. PubMed
  35. Baleriola J, et al. 2016. Sci Rep. 6: 25928. PubMed
  36. Shimada I, et al. 2012. J Neurosci. 32:7926-7940. PubMed
  37. Arber C, et al. 2019. Brain Commun. 1:fcz024. PubMed
  38. Jiang Y, et al. 2018. J Biol Chem. 293:19317. PubMed
  39. Stokowska A, et al. 2017. Brain. 140:353. PubMed
  40. Meng QT, et al. 2021. Elife. 10:. PubMed
  41. Olmsted ZT, et al. 2022. Front Cell Neurosci. 15:725195. PubMed
  42. Binder J, et al. 2022. Commun Biol. 5:125. PubMed
  43. Krishnan V, et al. 2016. J Neuropathol Exp Neurol. 75: 464 - 478. PubMed
  44. Brosig A, et al. 2019. Cell Rep. 29:2028. PubMed
  45. Girouard MP, et al. 2020. eneuro. 7(2):ENEURO. PubMed
  46. Vogt M, et al. 2021. Theranostics. 11:5569. PubMed
  47. Pires G, et al. 2021. Brain Commun. 3:fcab021. PubMed
  48. Nichterwitz S, et al. 2020. Genome Res. 30:1083. PubMed
  49. Brykczynska U, et al. 2016. Stem Cell Reports. 7:1059-1071. PubMed
  50. Omichi R, et al. 2020. Mol Ther Methods Clin Dev. 1.51875. PubMed
  51. Xie Y, et al. 2018. Stem Cell Res. 9:2010. PubMed
  52. Uezono N, et al. 2018. Stem Cells. 36:737. PubMed
  53. Tang SC, et al. 2017. Diabetologia. 61:158. PubMed
  54. MOSES C, et al. 2020. Sci Rep. 10:11393. PubMed
  55. Nayak G, et al. 2020. Cell Reports. 30(3):672-686.e8.. PubMed
  56. Dvoriantchikova G, et al. 2022. Sci Rep. 12:17152. PubMed
  57. Jo S, et al. 2021. Stem Cell Research. 56:102524. PubMed
  58. Gong C, et al. 2021. Cell Reports. 34(12):108889. PubMed
  59. Kurihara Y, et al. 2020. Journal of Neurochemistry. 155(3):285-299. PubMed
  60. Hastings RL, et al. 2020. Sci Rep. 10:11132. PubMed
  61. Hoffman LF, et al. 2018. J Assoc Res Otolaryngol. 19:33. PubMed
  62. Carlin D et al. 2018. eNeuro. 5(2) pii: ENEURO. PubMed
  63. Mertens J, et al. 2021. Cell Stem Cell. 28:1533. PubMed
  64. Boland M, et al. 2017. Brain. 10.1093/brain/aww357. PubMed
  65. Ou MY, et al. 2021. Cell Reports. 35(13):109290. PubMed
  66. Sakaguchi H, et al. 2019. Stem Cell Reports. 13:458. PubMed
  67. Voronova A et al. 2017. Neuron. 94(3):500-516 . PubMed
  68. Sabelström H, et al. 2019. Cell Rep. 28:2064. PubMed
  69. van Niekerk EA, et al. 2022. Cell Rep Methods. 2:100255. PubMed
  70. Avraham O, et al. 2021. Elife. 10:. PubMed
  71. Simone R, et al. 2021. Nature. 594:117. PubMed
  72. Lasagni Vitar RM, et al. 2021. Invest Ophthalmol Vis Sci. 62:26. PubMed
  73. Reddy A, et al. 2020. Cell. 183:62. PubMed
  74. Otani T, et al. 2016. Cell Stem Cell. 18: 467-480. PubMed
  75. Verstraelen P, et al. 2017. Front Cell Neurosci. 11:173. PubMed
  76. Kim H et al. 2017. Stem cell research. 26:72-75 . PubMed
  77. Li H, et al. 2019. iScience. 19:559. PubMed
  78. Macpherson P, et al. 2015. Development. 142: 4038 - 4048. PubMed
  79. Ferguson M, et al. 2013. Neurobiol Dis. 56:34-46. PubMed
  80. Yau K, et al. 2016. J Neurosci. 36: 1071 - 1085. PubMed
  81. Cui Q, et al. 2016. Nat Commun. 7:10637. PubMed
  82. Bosze B, et al. 2020. J Neurosci. 40:1501. PubMed
  83. Benoy V, et al. 2018. Brain. 10.1093/brain/awx375. PubMed
  84. Chabrat A, et al. 2019. Stem Cells Int. 2019:2945435. PubMed
  85. Yang C, et al. 2020. Neuron. 105:276. PubMed
  86. Suter TACS, et al. 2020. Cell Rep. 30:1164. PubMed
  87. Adams MT, et al. 2021. eLife. 0.416666666666667. PubMed
  88. Iannielli A, et al. 2019. Cell Rep. 29:4646. PubMed
  89. Bowles KR, et al. 2019. PLoS One. 14:e0213374. PubMed
  90. Toda T, et al. 2017. Cell Stem Cell. 21:618. PubMed
  91. Qiang L et al. 2018. Current biology : CB. 28(13):2181-2189 . PubMed
  92. Meijer M et al. 2019. Cell Rep. 27(7):2199-2211 . PubMed
  93. Finno CJ, et al. 2022. Front Cell Neurosci. 16:853035. PubMed
  94. Newland B, et al. 2021. ACS Chem Neurosci. 12:1178. PubMed
  95. Amador A, et al. 2020. Brain. 143:2039. PubMed
  96. Sugiyama T, et al. 2021. iScience. 24(7):102758. PubMed
  97. Bhaskaran S, et al. 2020. Aging Cell. 19:e13225. PubMed
  98. Rhee HJ et al. 2019. Cell Rep. 27(7):2212-2228 . PubMed
  99. Makar T, et al. 2018. Stem Cell Res Ther. 9:127. PubMed
  100. Zahr SK et al. 2018. Neuron. 97(3):520-537 . PubMed
  101. Cora V, et al. 2019. Cells. 8:5. PubMed
  102. Grove M, et al. 2020. eLife. 9:e50138.. PubMed
  103. Francistiová L, et al. 2022. Front Mol Neurosci. 14:793769. PubMed
  104. Iennaco R, et al. 2022. Cell Death Differ. 29:293. PubMed
  105. Bjorke B, et al. 2021. Dev Biol. 476:272. PubMed
  106. Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed
  107. Lin P, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G412 - G422. PubMed
  108. Tsueng G, et al. 2011. J Virol. 85:5718-5732. PubMed
  109. Alvarez–Lindo N, et al. 2019. Sci Rep. 9:18486. PubMed
  110. Tiwari D et al. 2019. Neurobiol Dis. 1871:48:00 . PubMed
  111. Carlin D et al. 2019. eNeuro. 6(3) pii: ENEURO. PubMed
  112. Li H, et al. 2018. Hum Mutat. 39:550. PubMed
  113. Smith LA, et al. 2021. Front Cell Dev Biol. 9:667246. PubMed
  114. Mao Y, et al. 2021. Cell Prolif. 54:e13090. PubMed
  115. Olmsted ZT, et al. 2022. iScience. 25:104486. PubMed
  116. Vahsen BF, et al. 2022. Sci Rep. 12:12606. PubMed
  117. Watanabe S, et al. 2016. EMBO Mol Med. 8(12):1421-1437. PubMed
  118. Cates K, et al. 2020. Cell Stem Cell. 28(1):127-140.e9. PubMed
  119. Ou J et al. 2018. Cell. 173(4):851-863 . PubMed
  120. Hsu J, et al. 2019. Nat Commun. 10:2939. PubMed
  121. Chanda S et al. 2019. Cell Stem Cell. 25(1):103-119 . PubMed
  122. Peitz M, et al. 2020. STAR Protoc. 1:100038. PubMed
  123. Flitsch LJ, et al. 2022. Sci Rep. 12:8356. PubMed
  124. Pahl MC, et al. 2021. Nat Commun. 12:6749. PubMed
  125. Kim H, et al. 2020. Sci Signal. 13: . PubMed
  126. Vicidomini C, et al. 2016. Mol Psychiatry. 10.1038/mp.2016.30. PubMed
  127. Barry DM, et al. 2020. Nat Commun. 11:1397. PubMed
  128. Hattori Y, et al. 2020. Nat Commun. 11:1631. PubMed
  129. Duncan DS, et al. 2018. Front Neurosci. 0.9875. PubMed
  130. Martínez JC et al. 2019. Neuron. 104(5):931-946 . PubMed
  131. Mickle AD, et al. 2019. Nature. 565:361. PubMed
  132. Kawakami Y et al. 2018. The Journal of Neuroscience. 38(10):2589-2604 . PubMed
  133. Flores-Otero J, et al. 2007. J Neurosci. 27:14023-14034. PubMed
  134. Zhang L, et al. 2016. Neuroscience. 337:88-97. PubMed
  135. Arber C, et al. 2021. Cell Reports. 34(2):108615. PubMed
  136. Shah K, et al. 2019. J Mol Cell Biol. N/A. PubMed
  137. Sar Shalom H, et al. 2019. Elife. 8:e41162. PubMed
  138. Facchin C, et al. 2020. Theranostics. 10:3518. PubMed
  139. Baker L, et al. 2021. JCI Insight. 6:. PubMed
  140. Besusso D, et al. 2020. Stem Cell Res. 102016:49. PubMed
  141. Shang Y, et al. 2018. J Neurosci. 38:4006. PubMed
  142. Jean-Louis T, et al. 2018. Neurobiol Aging. 62:130. PubMed
  143. Dan W, et al. 2022. Front Physiol. 12:744294. PubMed
  144. Avraham O, et al. 2022. Front Mol Neurosci. 14:796070. PubMed
  145. Jacob L, et al. 2022. J Exp Med. 219:. PubMed
  146. Arber C, et al. 2020. Mol Psychiatry. 25:2919. PubMed
  147. Smith-Anttila CJA, et al. 2021. Developmental Biology. 476:18-32. PubMed
  148. Hung C, et al. 2021. Cell Reports. 35(11):109259. PubMed
  149. Ornaghi F, et al. 2020. Neurobiol Dis. 134:104667. PubMed
  150. Hung COY et al. 2018. Cell reports. 25(13):3647-3660 . PubMed
  151. Jeon H, et al. 2018. J Clin Med. 7:. PubMed
  152. Avraham O, et al. 2022. Front Mol Neurosci. 15:967472. PubMed
  153. Su Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  154. Olmsted ZT, et al. 2021. iScience. 24(8):102827. PubMed
  155. Ward AI, et al. 2020. MBio. 11:00. PubMed
  156. Avraham O, et al. 2020. Nat Commun. 3.854861111. PubMed
  157. Anastasiadou S, Knöll B 2016. Exp Neurol. 279:243-260. PubMed
  158. Lee J, et al. 2018. J Clin Med. 7:. PubMed
RRID
AB_2564645 (BioLegend Cat. No. 802001)

Antigen Details

Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Gene ID
10381 View all products for this Gene ID
UniProt
View information about Tubulin beta-3 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 10.24.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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