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Purified anti-Tubulin-γ Antibody

Pricing & Availability
Clone
Poly6209 (See other available formats)
Regulatory Status
RUO
Other Names
γ-tubulin
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
1-Poly6209_Pure_Tubulin-gamma_Antibody_1_012419
Total cell lysates (15 µg total protein) from A549, U87-MG, NTERA-2, and U937 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:1000 dilution of Purified anti-Tubulin-γ Antibody, clone Poly6209, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 1-Poly6209_Pure_Tubulin-gamma_Antibody_1_012419
    Total cell lysates (15 µg total protein) from A549, U87-MG, NTERA-2, and U937 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:1000 dilution of Purified anti-Tubulin-γ Antibody, clone Poly6209, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 2-g-tubulin
    Whole cell extract from A431 cells (Lane 1) and NIH3T3 cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-γ-tubulin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Cat # Size Price Quantity Check Availability Save
620901 50 µL 89 CHF
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620902 200 µL 265 CHF
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Description

γ-tubulin is a 51 kD member of the tubulin superfamily and is conserved between yeast and mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to be involved in cytoskeletal organization and mitosis. γ-tubulin is expressed at highest levels during prophase to metaphase and can be modified by phosphorylation. This protein has been shown to interact with α-tubulin, paxillin, and RAC GTPase activated protein 1. The Poly6209 antibody has been shown to be useful for Western blotting of human and mouse γ-tubulin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide-KLH
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is a dilution of 1:1000 - 1:8000. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Additional Product Notes

The 50 µL size can be used for approximately 10 Western blots, and the 200 µL size can be used for approximately 40 Western blots.

Application References

(PubMed link indicates BioLegend citation)
  1. Fry AM, et al. 1998. EMBO J. 17:470.
  2. Zeng SR, et al. 2009. Mol Cell Biol. 112:1379. PubMed
  3. Solc P, et al. 2012. Biol Reprod. 87:1095. PubMed.
Product Citations
  1. Kowal TJ, et al. 2022. J Comp Neurol. 530:2176. PubMed
  2. Belaadi N, et al. 2022. Proc Natl Acad Sci U S A. 119:e2116167119. PubMed
  3. Baran V, et al. 2013. Mol Reprod Dev. 522:80. PubMed
  4. Zeng S, et al. 2009. Mol Cell Biol. 30:935. PubMed
  5. Solc P, et al. 2012. Biol Reprod. 87:1095. PubMed
RRID
SCR_001134 (BioLegend Cat. No. 620901)
SCR_001134 (BioLegend Cat. No. 620902)

Antigen Details

Structure
Tubulin superfamily, highly conserved between yeast and mammals. Predicted molecular weight approximately 51 kD
Distribution

Cytoskeletal protein, high expressed in centrosomes

Function
Cytoskeletal organization, mitosis
Interaction
Interacts with α-tubulin, paxillin, RAC GTPase activated protein 1
Modification
Phosphorylation
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microtubules
Antigen References

1. Zheng Y, et al. 1991. Cell 65:817.

Regulation
Expressed at highest levels during prophase to metaphase
Gene ID
2276 View all products for this Gene ID
UniProt
View information about Tubulin-gamma on UniProt.org
Go To Top Version: 3    Revision Date: 06.15.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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