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Purified anti-XPC Antibody

Pricing & Availability
Clone
W20162C (See other available formats)
Regulatory Status
RUO
Other Names
DNA repair protein complementing XP-C cells (XPC), Xeroderma pigmentosum complementation group C (XPC), p125, XPC-Complex Subunit
Isotype
Rat IgG2b, λ
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Product Citations
publications
W20162C_PURE_XPC_Antibody_040522
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-XPC (clone W20162C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker
  • W20162C_PURE_XPC_Antibody_040522
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL of purified anti-XPC (clone W20162C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:25000 dilution. Lane M: Molecular weight marker
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604851 25 µg 118 CHF
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604852 100 µg 335 CHF
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Description

XPC is a protein involved in the nucleotide excision repair (NER) pathway, a mechanism that repairs single-stranded DNA damage. XPC is a member of the XPC complex that recognizes DNA damage resulting in thermodynamically unstable DNA structures. For example, the unstable structures caused by the damage from ultraviolet light. XPC-RAD23B activation recruits other NER pathway members to the site and promotes progression through NER. XPC mutations can result in the disease that is the protein’s namesake, Xeroderma pigmentosum, characterized by an extreme sensitivity to sunlight and high cancer risk. More broadly speaking, since DNA damage underlies many types of cancer, changes in XPC expression levels or mutations in XPC are often found in tumors. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human XPC
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product does not cross-react with mouse by WB.

This product is not recommended for ICC and IHC-P. 

RRID
AB_2924610 (BioLegend Cat. No. 604851)
AB_2924610 (BioLegend Cat. No. 604852)

Antigen Details

Structure
XPC is a 940 amino-acid protein with a predicted molecular weight of 106 kD.
Distribution

XPC is expressed in most mammalian cells, shuttling between the nucleus and cytoplasm in the absence of DNA damage. 

Function
XPC binds DNA in the presence of DNA damage
Interaction
XPC forms the XPC complex along with RAD23B and CETN2
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, DNA Repair/Replication
Molecular Family
Nuclear Markers
Antigen References
  1. Nezow L, et al. 2015. DNA Repair. 26:19-27.
  2. Sugasawa K, 2008. Adv Exp Med Biol. 637:47-56. 
Gene ID
7508 View all products for this Gene ID
UniProt
View information about XPC1 on UniProt.org
Go To Top Version: 1    Revision Date: 04.05.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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