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Purified anti‐GFAP (Cocktail)

Pricing & Availability
Clone
SMI 22 (See other available formats)
Regulatory Status
RUO
Other Names
Glial fibrillary acidic protein
Isotype
Mouse IgG2b
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Product Citations
publications
SMI22_pure_GFAP_Antibody_1_031518
IHC staining of purified anti‐GFAP (Cocktail) antibody (clone SMI 22) on formalin-fixed paraffin-embedded human (left) and rat (right) brain tissues. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissues were incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • SMI22_pure_GFAP_Antibody_1_031518
    IHC staining of purified anti‐GFAP (Cocktail) antibody (clone SMI 22) on formalin-fixed paraffin-embedded human (left) and rat (right) brain tissues. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissues were incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • SMI22_pure_GFAP_Antibody_2_031518
    IHC staining of purified anti‐GFAP (Cocktail) antibody (clone SMI 22) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 10 µg/mL of the primary antibody for 60 minutes at room temperature, followed by incubation with Alexa Fluor® 594 anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with 40 X objective. Scale bar: 50 µm
  • SMI22_pure_GFAP_Antibody_3_031518
    IHC staining of purified anti‐GFAP (Cocktail) antibody (clone SMI 22) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 10 µg/mL of the primary antibody for 60 minutes at room temperature, followed by incubation with Alexa Fluor® 594 anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with 40 X objective. Scale bar: 50 µm
  • SMI22_pure_GFAP_Antibody_4_031518
    IHC staining of purified anti‐GFAP (Cocktail) antibody (clone SMI 22) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with 10 µg/mL of the primary antibody for 60 minutes at room temperature, followed by incubation with Alexa Fluor® 594 anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with 40 X objective. Scale bar: 50 µm
  • SMI22_pure_GFAP_Antibody_5_031518
    ICC staining of purified anti‐GFAP (Cocktail) antibody (clone SMI 22) on U251 cells. The cells were fixed with ice cold methanol, permeabilized with a buffer containing 0.1% Triton X-100 and 0.1% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation wit 2.5 µg/ml of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm Image
  • SMI22_pure_GFAP_Antibody_6_031518
    Western blot of purified anti‐GFAP (Cocktail) antibody (clone SMI 22). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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835303 25 µg 112 CHF
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835304 100 µg 276 CHF
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Description

Glial fibrillary acidic protein (GFAP) is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts, Leydig cells of the testis, keratinocytes, osteocytes and chondrocytes and stellate cells of the pancreas and liver. GFAP is a type III IF protein that is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells.

Type III intermediate filaments are highly conserved and contain three domains, named the head, rod and tail domains. This rod domain coils around that of another filament to form a dimer, with the N-terminal and C-terminal of each filament aligned. Type III filaments such as GFAP are capable of forming both homodimers and heterodimers; GFAP can polymerize with other type III proteins or with neurofilament light chain protein (NF-L). Interestingly, GFAP and other type III IF proteins cannot assemble with keratins, the type I and II intermediate filaments: in cells that express both proteins, two separate intermediate filament networks form.

To form networks, the initial GFAP dimers combine to make staggered tetramers, which are the basic subunits of an intermediate filament. The non-helical head and tail domains are necessary for filament formation. The head and tail regions have greater variability of sequence and structure. In spite of this increased variability, the head of GFAP contains two conserved arginines and an aromatic residue that are required for proper assembly.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Chicken
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Multiple protein fragments ranging from 38 to 48 kD have been reported in human CNS lysates resulting from caspase- and calpain-mediated cleavage of GFAP.

RRID
AB_2728544 (BioLegend Cat. No. 835303)
AB_2728544 (BioLegend Cat. No. 835304)

Antigen Details

Structure
GFAP is a 432 amino acid protein with a molecular mass of ~50 kD.
Distribution

Tissue distribution: GFAP is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, ependymal cells, and Bergmann glia cells (protoplasmic astrocyte). GFAP is expressed in cells lacking fibronectin.
Cellular distribution: Cytoskeleton and cytosol

Function
GFAP is a class-III intermediate filament and a structural constituent of the cytoskeleton. It is a cell-specific marker that is used to distinguish astrocytes from other glial cells during the development of the CNS.
Cell Type
Astrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org
Go To Top Version: 3    Revision Date: 09.12.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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