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RAPID MAX™ Human Leptin ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Obese Protein, Obesity Factor, LEPD, OB, OBS
Ave. Rating
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Product Citations
publications
Rapid-Max_Human-Leptin-ELISA-Kit_030624
  • Rapid-Max_Human-Leptin-ELISA-Kit_030624
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444309 1 Pre-coated Plate 595 CHF
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Description

Human Leptin is a 167 amino acid protein and approximately 16 kD in mass. Leptin is encoded by the obese (ob) gene and the gene locates on chromosome 7. Leptin is an adipocyte-derived hormone and mainly secreted by adipocyte; small amounts of Leptin are expressed by the cells in the epithelium of the stomach and in the placenta. Leptin’s effects on body weight are mediated through impacts on hypothalamic center that control feeding behavior, hunger, body temperature, and energy expenditure. Leptin importantly regulates food intake, body weight, metabolism, energy expenditure, reproductive function, and adiposity through hypothalamic Leptin receptor. Leptin is also the member of long-chain helical cytokine family. 

The BioLegend RAPID MAX™ Human Leptin ELISA Kit is a one step, one wash, 70-minute enzyme-linked immunosorbent sandwich assay (ELISA) with a pre-coated, 96-well strip plate that immobilizes the biotinylated capture antibody. Standards and samples are added into the wells, followed by an antibody cocktail. The antibody cocktail consists of a biotinylated monoclonal capture antibody and an HRP-conjugated monoclonal detection antibody. This kit is specifically designed for the accurate quantitation of human Leptin cell culture supernatant, serum, plasma. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • RAPID MAX™ Pre-coated 96-well Strip Microplate
  • Human Leptin Capture Antibody Concentrate (200X)
  • Human Leptin Detection Antibody Concentrate (200X)
  • Human Leptin Standard
  • Assay Diluent B (5X), 12 mL
  • Substrate Solution F
  • Wash Buffer (20X)
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA
Sensitivity
19.6 ± 2.4 pg/mL
Standard Range
62.5 - 4000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Type
Endothelial cells, Fibroblasts
Biology Area
Angiogenesis, Cell Biology, Signal Transduction
Molecular Family
Enzymes and Regulators
Gene ID
NA
UniProt
View information about Leptin on UniProt.org
Go To Top Version: 1    Revision Date: 03.06.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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