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- Regulatory Status
- RUO
- Other Names
- Angiopoietin-related protein 3, Angiopoietin-5 (ANG-5),Angiopoietin-like protein 3
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- Product Citations
- publications
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Recombinant human ANGPTL-3 binds to integrin αvβ3 in a dose-dependent manner. EC50 of this effect is 1 – 7 µg/mL. -
Stability testing for recombinant human ANGPTL-3. Recombinant human ANGPTL-3 was aliquoted in PBS, pH 7.2, at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to bind to integrin αvβ3 in a dose-dependent manner. The EC50 for this effect is 1 – 7 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 594854 | 25 µg | 195 CHF | ||||
| 594856 | 100 µg | 315 CHF | ||||
A family of proteins that is structurally similar to the angiopoietins has been identified as angiopoietin-like proteins (ANGPTLs). To date, eight ANGPTLs have been discovered, namely ANGPTL1 to ANGPTL8. All ANGPTLs contain an amino-terminal coiled-coil domain, a linker region, and a carboxy-terminal fibrinogen-like domain. ANGPTLs have been generally considered orphan ligands as they do not bind to the receptors classically targeted by angiopoietins, namely the tyrosine kinase with immunoglobulin-like and EGF-like domain 1 (Tie1) and the endothelialspecific receptor tyrosine kinase (TEK or Tie2). ANGPTL3 is a main regulator of lipoprotein metabolism as it is directly involved in inhibition of lipoprotein lipase (LPL) activity. ANGPTL3 deficient mice exhibit low plasma HDL cholesterol and HDL phospholipid (PL). In humans, the complete absence of ANGPTL3 results in an increased LPL activity and mass and lower circulating free fatty acid levels. The physical association of ANGPTL3 with ANGPTL8 greatly increases its affinity for LPL and creates a very potent endocrine inhibitor of plasma triglyceride clearance in heart, skeletal muscle and brown adipose tissue. The identification of integrin αvβ3 as a receptor for ANGPTL3 on the vasculature further help in dissecting its role in lipid metabolism and inflammatory diseases. Additional functions of ANGPTL3 has been highlighted in literature as inducer of actin filament rearrangement in glomerular podocytes and be a promising non-invasive biomarker in diagnosis of chronic hepatitis.
Product DetailsProduct Details
- Source
- Human ANGPTL-3, amino acids Ser17 - Glu460 (Accession # Q9Y5C1), with a C-terminal 8His tag, was expressed in CHO cells.
- Molecular Mass
- The 457 amino acid recombinant protein has a predicted molecular mass of approximately 53.3 kD. The DTT-reduced and non-reduced proteinmigrate at approximately 60 - 70 kD in SDS-PAGE.
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N-terminal
Sequence Analysis - The predicted N-terminal amino acid is Ser.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per μg of cytokine as determined by the LAL method
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human ANGPTL-3 binds to integrin αvβ3 in a dose-dependent manner. EC50 of this effect is 1 – 7 µg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Dimer
- Function
- Lipoprotein metabolism regulation, inhibition of lipoprotein lipase (LPL) activity and angiogenesis
- Interaction
- Podocytes, vascular endothelial cells
- Ligand/Receptor
- Integrin αvβ3 on vasculature
- Bioactivity
- Recombinant human ANGPTL-3 binds to integrin αvβ3 in a dose-dependent manner. EC50 of this effect is 1 – 7 µg/mL.
- Cell Type
- Endothelial cells
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology
- Molecular Family
- Soluble Receptors
- Antigen References
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- Kim I, et al. 1999. FEBS Lett. 443:353-6.
- Ward NL, et al. 2002. Semin Cell Dev Biol. 13:19-27.
- Ono M, et al. 2003. J BiolChem. 278:41804.
- Jin W, et al. 2007. Cell Metab. 6:129-36.
- Robciuc MR, et al. 2013. Arterioscler Thromb Vasc Biol. 33:1706-13.
- Shimamura M, et al. 2007. ArteriosclerThrombVasc Biol. 27:366.
- Oldoni F, et al. 2020. JCI insight. 5:e138777.
- Camenisch G, et al. 2002. J Biol Chem. 277:17281-90.
- Lin Yi, et al. 2013. Biomed Res Int. 2013:135608.
- Gene ID
- 27329 View all products for this Gene ID
- UniProt
- View information about ANGPTL-3 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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