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- Regulatory Status
- RUO
- Other Names
- EPH Receptor A1, Erythropoietin-Producing Hepatoma Receptor, Tyrosine-Protein Kinase Receptor EPH, Ephrin Type-A Receptor 1, EC 2.7.10.1, HEpha1, EPHT1, EPHT, EPH, Erythropoietin-Producing Hepatoma Amplified Sequence
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- Product Citations
- publications
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Immobilized recombinant human EphA1-Fc Chimera binds biotinylated recombinant human Ephrin-A1-Fc Chimera in a dose-dependent manner with an EC50 range of 5 - 20 ng/mL. -
Stability Testing for Recombinant Human EphA1-Fc Chimera. Recombinant human EphA1-Fc Chimera was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with biotinylated recombinant human Ephrin-A1-Fc Chimera.
The Eph family of receptor tyrosine kinases (RTKs) are comprised of nine EphA receptors (A1-A8 and A10) as well as five EphB receptors (B1-B4 and B6). The interaction between Eph receptors and their Ephrin ligands generates bidirectional signaling that affects both receptor and ligand expressing cells. Mature EphA1 receptors contain a 524 amino acid extracellular domain (ECD) comprised of a globular domain on the N-terminal and a cysteine-rich region followed by two fibronectin type III domains, a 21 amino acid transmembrane segment, and a 408 amino acid cytoplasmic domain that includes a juxtamembrane motif, a conserved sterile alpha motif, and a tyrosine kinase domain. EphA1 is expressed on CD4+ T cells where it promotes migration via tyrosine phosphorylation of PYK2. It is also expressed in differentiated epithelial cells of the skin, thymus, kidney, and adrenal cortex. EphA1 contributes to the invasiveness of many cancers by altering their adhesion, motility, and angiogenesis. Abnormal EphA1 expression or function has been characterized in cancers of the lungs, stomach, ovaries, and bladder.
Product DetailsProduct Details
- Source
- Human EphA1, amino acid Lys26-Glu547 (Accession # NM_005232.4) with C-terminal human IgG Fc tag was expressed in CHO cells.
- Molecular Mass
- The 761 amino acid recombinant protein has a predicted molecular mass of approximately 83.5 kD. The DTT-reduced protein migrates at approximately 100 kD and the non-reduced protein migrates at approximately 200 kD by SDS-PAGE. The predicted N-terminal amino acid is Lys.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When recombinant human EphA1-Fc Chimera is immobilized at 5 µg/mL, biotinylated recombinant human Ephrin-A1-Fc Chimera binds in a dose-dependent manner. The EC50 range for this effect is 5 - 20 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide linked homodimer
- Distribution
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Widely expressed in differentiated epithelial cells in bone marrow, spleen, thymus, and testes. Also expressed on CD4+ T cells and a subpopulation of CD19+ B cells
- Function
- Receptor tyrosine kinase which binds promiscuously membrane-bound Ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells
- Interaction
- Epithelial cells, Endothelial cells
- Ligand/Receptor
- Ephrin-A1 (EFNA1), EFNA3, EFNA4
- Bioactivity
- Measured by its ability to bind recombinant human Ephrin-A1
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Antigen References
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- Lisabeth EM, et al. 2013. Cold Spring Harb Perspect Biol. USA. 5:9.
- Pasquale EB. 2008. Cell. 133:38-52.
- Hirai H, et al. 1987. Science. 238:1717-20.
- Aasheim HC, et al. 2005. Blood. 105:2869-76.
- Coulthard MG, et al. 2001. Growth Factors. 18:303-17.
- Ieguchi K and Maru Y. 2019. Cancer Sci. 3:841.
- Inokuchi M, et al. 2018. Anticancer Res. 3:1685
- Jin Y, et al. 2018. Mol. Med. Rep. 17:5409.
- Chu M and Zhang C. 2018. Sci Rep. 1:1539
- Gene ID
- 2041 View all products for this Gene ID
- UniProt
- View information about EphA1 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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