Recombinant Human EphB2-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
EPH Receptor B2, Developmentally-Regulated Eph-Related Tyrosine Kinase, Tyrosine-Protein Kinase Receptor EPH-3, Renal Carcinoma Antigen NY-REN-47, Tyrosine-Protein Kinase TYRO5, Ephrin Type-B Receptor 2, EPH-Like Kinase 5, EC 2.7.10.1, EPHT3, HEK5
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Human_EphB2-Fc_Chimera_CF_1_042820.png
When recombinant human Ephrin-B2 is immobilized at 2 µg/mL, recombinant human EphB2-Fc Chimera binds in a dose-dependent manner. The EC50 range for this effect is 10 - 40 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
  • Human_EphB2-Fc_Chimera_CF_1_042820.png
    When recombinant human Ephrin-B2 is immobilized at 2 µg/mL, recombinant human EphB2-Fc Chimera binds in a dose-dependent manner. The EC50 range for this effect is 10 - 40 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
  • Human_EphB2-Fc_Chimera_CF_2_042820.png
    Stability Testing for Recombinant Human EphB2-Fc Chimera. Recombinant human EphB2-Fc Chimera was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with recombinant human Ephrin-B2.
Cat # Size Price Quantity Check Availability Save
791702 10 µg 83 CHF
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791704 25 µg 129 CHF
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791706 100 µg 323 CHF
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791708 500 µg 762 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

The Eph family of receptor tyrosine kinases (RTKs) are comprised of nine EphA receptors (A1-A8 and A10) as well as five EphB receptors (B1-B4 and B6). The interaction between Eph receptors and their Ephrin ligands generates bidirectional signaling that affects both receptor and ligand expressing cells. Mature EphA1 receptors contain a 524 amino acid extracellular domain (ECD) comprised of a globular domain on the N-terminal and a cysteine-rich region followed by two fibronectin type III domains, a 21 amino acid transmembrane segment, and a 408 amino acid cytoplasmic domain that includes a juxtamembrane motif, a conserved sterile alpha motif, and a tyrosine kinase domain. EphA1 is expressed on CD4+ T cells where it promotes migration via tyrosine phosphorylation of PYK2. It is also expressed in differentiated epithelial cells of the skin, thymus, kidney, and adrenal cortex. EphA1 contributes to the invasiveness of many cancers by altering their adhesion, motility, and angiogenesis. Abnormal EphA1 expression or function has been characterized in cancers of the lungs, stomach, ovaries, and bladder.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human EphB2, amino acid Val19-Leu543 (Accession # NM_004442.6) with C-terminal human IgG Fc tag was expressed in CHO cells.
Molecular Mass
The 764 amino acid recombinant protein has a predicted molecular mass of approximately 85 kD. The DTT-reduced protein migrates at approximately 100 kD and the non-reduced protein migrates at approximately 200 kD by SDS-PAGE. The predicted N-terminal amino acid is Val.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When recombinant human Ephrin-B2 is immobilized at 2 µg/mL, recombinant human EphB2-Fc Chimera binds in a dose-dependent manner. The EC50 range for this effect is 10 - 40 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Disulfide linked homodimer
Distribution

Widely expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain

Function
Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex.
Interaction
Neurons
Ligand/Receptor
Ephrin-B1, Ephrin-B2
Bioactivity
Measured by its ability to bind recombinant human Ephrin-B2
Cell Type
Endothelial cells, Neurons
Biology Area
Angiogenesis, Cell Motility/Cytoskeleton/Structure, Neuroscience, Synaptic Biology
Molecular Family
Soluble Receptors
Antigen References
  1. Lisabeth EM, et al. 2013. Cold Spring Harb Perspect Biol. 5(9)
  2. Pasquale EB. 2008. Cell. 133:38.
  3. Hirai H, et al. 1987. Science. 238:1717-20.
  4. Aasheim HC, et al. 2005. Blood. 105:2869-76.
  5. Coulthard MG, et al. 2001. Growth Factors. 18:303-17.
  6. Ieguchi K and Maru Y. 2019. Cancer Sci. 110:841.
  7. Inokuchi M, et al. 2018. Anticancer Res. 38:1685
  8. Jin Y, et al. 2018. Mol. Med. Rep. 17:5409.
  9. Chu M, et al. 2018. Sci. Rep. 1:1539.
Gene ID
2048 View all products for this Gene ID
UniProt
View information about EphB2 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 04.28.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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