- Regulatory Status
- RUO
- Other Names
- FYN Proto-Oncogene, Src Family Tyrosine Kinase, SLK, SYN, P59-Fyn, EC 2.7.10, Tyrosine-protein kinase Fyn, Src-Like Kinase,Protein-tyrosine kinase fyn, Proto-oncogene tyrosine-proteinkinase fyn, Tyrosine kinase p59fyn
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- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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588906 | 100 µg | 300 CHF |
Fyn is a 59 kDa protein tyrosine kinase and a member of the Src subfamily. Two isoforms of Fyn have been reported: FynT, found in T cells, B cells, and thymocytes, and FynB, found in neuronal cells. Fyn is expressed in various cancers, as well as in neurons and glial cells, playing a crucial role in brain development and regulating neuronal migration. Fyn is also associated with TCR signaling, cytoskeletal reorganization, and IL-4 production. Fyn-deficient mice exhibit significantly reduced numbers of NKT cell. It has been shown to regulate cell growth, interact with the p85 subunit of PI3K, control high-affinity IgE receptor-mediated mast cell degranulation, and participate in neuronal cell signaling. Fyn can be activated by T cell receptor engagement or anti-CD3 ligation and undergoes modifications through phosphorylation and palmitoylation. It interacts with Fyn-binding protein, p85 PI3K, the high-affinity IgE receptor, and NCAM140. In cancer, Fyn promotes tumor growth and metastasis through its roles in cell growth, apoptosis, motility, migration, and the development of drug resistance in various tumors.
Product DetailsProduct Details
- Source
- Human FYN, amino acids (Met1-Leu537) (Accession #P06241) with a N-terminal 6His-GST-TEV tag was expressed in insect cells.
- Molecular Mass
- The 780 amino acid recombinant protein has a predicted molecular mass of approximately 89.0 kD. The DTT-reduced and non-reduced protein migrates at approximately 70-85 kD by SDS-PAGE. The N-terminal amino acid is Met.
- Purity
- > 85% as determined by Coomassie stained SDS-PAGE.
- Formulation
- Protein is formulated in 50 mM Tris, 150 mM NaCl, 25% Glycerol, 1 mM TCEP, 1 mM EDTA, 0.1 mM PMSF, pH 7.5
- Concentration
- 100 µg at 0.5 mg/mL
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 1 week, at -20°C for up to three months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Application
-
Standard
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Protein tyrosine kinase family
- Distribution
-
T cells, thymocytes, B cells, neuronal cells
- Function
- Controls cell growth, interacts with p85 subunit of PI3K; involved in high affinity IgE receptor-mediated mast cell degranulation
- Interaction
- Fyn binding protein, p85 PI3K, high affinity IgE receptor, NCAM140
- Cell Type
- B cells, Neurons, T cells, Thymocytes
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Immuno-Oncology, Neurodegeneration, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
- Doi H, et al. 2014. Clin Immunol. 184:184-91.
- Nelson MH, et al. 2015. J Immunol. 1737:1737-47.
- Wu L, et al. 2023. Cell Rep Med. 4:100917.
- Shi W, et al. 2020. J Biol Chem. 295:7653-7668.
- Dwyer A, et al. 2016. J Leukoc Biol. 100:163.
- Laird R, et al. 2010. J Immunol. 185:6518.
- Laird R, Hayes S 2010. PLoS One. 5:e8899.
- Lautz JD, et al. 2021. Cell Rep. 37:110076.
- Renatta K and Xiangning J. 2015. Dev Neurosci. 37:311.
- Gene ID
- 2534 View all products for this Gene ID
- UniProt
- View information about FYN on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
-
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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