Recombinant Human FYN (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
FYN Proto-Oncogene, Src Family Tyrosine Kinase, SLK, SYN, P59-Fyn, EC 2.7.10, Tyrosine-protein kinase Fyn, Src-Like Kinase,Protein-tyrosine kinase fyn, Proto-oncogene tyrosine-proteinkinase fyn, Tyrosine kinase p59fyn
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Product Citations
publications
Recom_Human-FYN_1_CF_103124
Stability Testing for Recombinant Human FYN. One aliquot (Control) was stored at 4°C for 7 days, while another aliquot (4XF/T) was subjected to multiple freeze-thaw cycles by freezing at -80°C and thawing four times over a 7-day period. Each freeze-thaw cycle involved placing the sample on ice and slowly thawing it until no ice crystals remained in the tube. A minimum of 24 hours elapsed between each freeze and thaw event. Following treatment, the reduced samples (4 µg/lane) were separated by SDS-PAGE and stained with Coomassie brilliant blue dye. Lane M: Protein ladder (Cat. No. 773302).
  • Recom_Human-FYN_1_CF_103124
    Stability Testing for Recombinant Human FYN. One aliquot (Control) was stored at 4°C for 7 days, while another aliquot (4XF/T) was subjected to multiple freeze-thaw cycles by freezing at -80°C and thawing four times over a 7-day period. Each freeze-thaw cycle involved placing the sample on ice and slowly thawing it until no ice crystals remained in the tube. A minimum of 24 hours elapsed between each freeze and thaw event. Following treatment, the reduced samples (4 µg/lane) were separated by SDS-PAGE and stained with Coomassie brilliant blue dye. Lane M: Protein ladder (Cat. No. 773302).
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588906 100 µg 300 CHF
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Description

Fyn is a 59 kDa protein tyrosine kinase and a member of the Src subfamily. Two isoforms of Fyn have been reported: FynT, found in T cells, B cells, and thymocytes, and FynB, found in neuronal cells. Fyn is expressed in various cancers, as well as in neurons and glial cells, playing a crucial role in brain development and regulating neuronal migration. Fyn is also associated with TCR signaling, cytoskeletal reorganization, and IL-4 production. Fyn-deficient mice exhibit significantly reduced numbers of NKT cell. It has been shown to regulate cell growth, interact with the p85 subunit of PI3K, control high-affinity IgE receptor-mediated mast cell degranulation, and participate in neuronal cell signaling. Fyn can be activated by T cell receptor engagement or anti-CD3 ligation and undergoes modifications through phosphorylation and palmitoylation. It interacts with Fyn-binding protein, p85 PI3K, the high-affinity IgE receptor, and NCAM140. In cancer, Fyn promotes tumor growth and metastasis through its roles in cell growth, apoptosis, motility, migration, and the development of drug resistance in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human FYN, amino acids (Met1-Leu537) (Accession #P06241) with a N-terminal 6His-GST-TEV tag was expressed in insect cells.
Molecular Mass
The 780 amino acid recombinant protein has a predicted molecular mass of approximately 89.0 kD. The DTT-reduced and non-reduced protein migrates at approximately 70-85 kD by SDS-PAGE. The N-terminal amino acid is Met.
Purity
> 85% as determined by Coomassie stained SDS-PAGE.
Formulation
Protein is formulated in 50 mM Tris, 150 mM NaCl, 25% Glycerol, 1 mM TCEP, 1 mM EDTA, 0.1 mM PMSF, pH 7.5
Concentration
100 µg at 0.5 mg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 1 week, at -20°C for up to three months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Application

Standard

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Protein tyrosine kinase family
Distribution

T cells, thymocytes, B cells, neuronal cells

Function
Controls cell growth, interacts with p85 subunit of PI3K; involved in high affinity IgE receptor-mediated mast cell degranulation
Interaction
Fyn binding protein, p85 PI3K, high affinity IgE receptor, NCAM140
Cell Type
B cells, Neurons, T cells, Thymocytes
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Immuno-Oncology, Neurodegeneration, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Doi H, et al. 2014. Clin Immunol. 184:184-91.
  2. Nelson MH, et al. 2015. J Immunol. 1737:1737-47.
  3. Wu L, et al. 2023. Cell Rep Med. 4:100917.
  4. Shi W, et al. 2020. J Biol Chem. 295:7653-7668.
  5. Dwyer A, et al. 2016. J Leukoc Biol. 100:163.
  6. Laird R, et al. 2010. J Immunol. 185:6518.
  7. Laird R, Hayes S 2010. PLoS One. 5:e8899.
  8. Lautz JD, et al. 2021. Cell Rep. 37:110076.
  9. Renatta K and Xiangning J. 2015. Dev Neurosci. 37:311.
Gene ID
2534 View all products for this Gene ID
UniProt
View information about FYN on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

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For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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