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- Regulatory Status
- RUO
- Other Names
- Auxillin-2, Auxillin 2, Auxillin II, cyclin G associated kinase, cyclinG-associated kinase, EC 2.7.11.1, GAK, DNAJ26
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Stability Testing for Recombinant Human GAK. One aliquot (Control) was stored at 4°C for 7 days, while another aliquot (4XF/T) was subjected to multiple freeze-thaw cycles by freezing at -80°C and thawing four times over a 7-day period. Each freeze-thaw cycle involved placing the sample on ice and slowly thawing it until no ice crystals remained in the tube. A minimum of 24 hours elapsed between each freeze and thaw event. Following treatment, the reduced samples (4 µg/lane) were separated by SDS-PAGE and stained with Coomassie brilliant blue dye. Lane M: Protein ladder (Cat. No. 773302)
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 568906 | 100 µg | 300 CHF | ||||
Cyclin G-associated kinase (GAK), also known as auxilin 2, is a serine/threonine protein kinase that plays a crucial role in cellular processes, including membrane trafficking and cell cycle regulation. GAK contains distinct domains, including a Ser/Thr protein kinase domain, a phosphatase tensin/auxilin domain, a PTEN C2 domain, and a J domain. GAK is widely expressed in various cells and tissues. GAK plays a key role in clarthrin-mediated endocytosis via the interaction of the heat shock cognate protein 70. GAK regulates cyclin-dependent kinases. GAK is also involved in the regulation of protein degradation pathways, including the ubiquitin-proteasome system. GAK has emerged as a gene associated with Parkinson's disease.
Product DetailsProduct Details
- Source
- Human GAK, amino acids (Gly13-Val351) (Accession# O14976-1) with a N-terminal 6His tag, was expressed in insect cells
- Molecular Mass
- The 352 amino acid recombinant protein has a predicted molecular mass of approximately 39.72 kD. The DTT-reduced and non-reduced protein migrates at approximately 31-50 kD by SDS-PAGE.
- Purity
- > 85% as determined by Coomassie stained SDS-PAGE.
- Formulation
- Protein is formulated in 50 mM HEPES, 150 mM NaCl, 25% glycerol, 1mM TCEP, pH 7.5
- Concentration
- 100 µg at 0.5 mg/mL
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 1 week, at -20°C for up to three months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Application
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Standard
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped with dry ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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Broadly distributed in normal adult tissues including brain, Lymphoid, lung, gastrointestinal tract, kidney, female reproduction system, and kidney
- Function
- Clathrin-mediated endocytosis, Membrane Trafficking, Vesicle-mediated transport
- Interaction
- Androgen receptor
- Cell Type
- Endothelial cells, Epithelial cells, Lymphocytes, Mesenchymal cells
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Neurodegeneration, Neuroscience, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase, Synaptic Vesicle Trafficking/Endocytosis
- Antigen References
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- Shimizu H, et al. 2009. J Cell Sci. 122:3145-52.
- Tanenbaum ME, et al. 2010. Chromosoma. 119:415-24.
- Dong-Won L, et al. 2005. J Cell Sci. 118:4311.
- Dong-Won L, et al. 2006. J Cell Sci. 119:3502.
- Pickrell JK, et al. 2016. Nat Genet. 48: 709-17.
- Yang-In Y, et al. 2010. Proc Natl Acad Sci USA. 76:89-92.
- Gene ID
- 2580 View all products for this Gene ID
- UniProt
- View information about GAK on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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