Recombinant Human LAIR2-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
CD306, CD306 antigen, leukocyte-associated Ig-like receptor 2;leukocyte associated immunoglobulin like receptor 2, MGC71634
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Product Citations
publications
Recom_Human-LAIR2-Fc-Chimera_1_CF
Recombinant Human LAIR2-Fc Chimera binds to immobilized collagen type I in a dose-dependent manner. The ED50 for this effect is 1.2 - 8.4 ng/mL.
  • Recom_Human-LAIR2-Fc-Chimera_1_CF
    Recombinant Human LAIR2-Fc Chimera binds to immobilized collagen type I in a dose-dependent manner. The ED50 for this effect is 1.2 - 8.4 ng/mL.
  • Recom_Human-LAIR2-Fc-Chimera_2_CF
    Stability testing for Recombinant Human LAIR2-Fc Chimera. Recombinant Human LAIR2-Fc Chimera was aliquoted in PBS, pH 7.2 with 10% glycerol, at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to bind to collagen type I in a dose-dependent manner. The ED50 for this effect is 1.2 - 8.4 ng/mL.
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567604 25 µg 295 CHF
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567606 100 µg 625 CHF
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Description

LAIR2 (leukocyte associated immunoglobulin like receptor 2) is a member of the immunoglobulin superfamily and a natural soluble homolog of LAIR1 that is present in only humans and non-human primates. LAIR2 shares 84% identity with the LAIR1 extracellular region, binds to collagens with higher affinity than LAIR1 and functions as a natural inhibitor to block cell membrane LAIR1’s inhibitory signaling. LAIR1 maps to a region of 19q13.4 in human chromosome, termed the leukocyte receptor cluster, which contains 29 genes in the immunoglobulin superfamily. Amount of soluble LAIR1 and LAIR2 are significantly increased in urine samples of RA patients and significantly correlates with inflammation markers. In addition, LAIR2 may also inhibit collagen-induced platelet aggregation and vessel formation during placental implantation. In vitro stimulation of sorted peripheral blood cells revealed that CD4+ T cells are the main producers of LAIR2. LAIR1 and LAIR2 bind to the collagen-like regions of C1q and of MBL (mannose binding lectin) and can efficiently inhibit complement activation via classical and lectin pathways. LAIR2 could inhibit tumor metastasis, invasion, and collagen deposition via suppressing TGF-β signaling suggesting that LAIR2 might act as a tumor suppressor.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human LAIR2-Fc Chimera, amino acids Gln22-Pro152 (Accession # Q6ISS4), with a C-terminal Fc tag, was expressed in 293E cells.
Molecular Mass
The 368 amino acid recombinant protein has a predicted molecular mass of approximately 40.7 kD. The DTT-reduced and non-reduced protein migrates at approximately 45-55 kD and 75-85 kD respectively in SDS-PAGE. The predicted N-terminal amino acid is Gln.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2 with 10% glycerol
Endotoxin Level
Less than 0.1 EU per μg of cytokine as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Recombinant human LAIR2-Fc Chimera binds to immobilized collagen type I in a dose-dependent manner. EC50 of this binding is 1.2 – 8.4 ng/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer
Distribution

Expressed on PBMCs and CD4+ T cells

Function
Blocks inhibitory signaling induced by LAIR1, tumor suppression and complement activation
Ligand/Receptor
Collagens and collagen-like regions of C1q and of MBL (mannose binding lectin)
Bioactivity
Human LAIR2-Fc Chimera binds to immobilized collagen type I in a dose-dependent manner. EC50 of this binding is 1.2 – 8.4 ng/mL.
Cell Type
T cells
Biology Area
Angiogenesis, Cell Adhesion, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Lebbink RJ, et al. 2008. Journal of Immunology. 180:1662-9.
  2. Lenting PJ, et al. 2010. PLoS One. 8:e12174.
  3. Olde Nordkamp MJ, et al. 2011. Arthritis and Rheumatism. 63:3749-57.
  4. Olde Nordkamp MJ, et al. 2014. J. Innate Immun. 6:284-92.
  5. Wang P, et al. 2023. Clinical and Experimental Immunology.
Gene ID
3904 View all products for this Gene ID
UniProt
View information about LAIR2 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 12.29.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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