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- Regulatory Status
- RUO
- Other Names
- Gelatinase B, Type IV collagenase, 92 kD gelatinase, Type V collagenase, MMP
- Ave. Rating
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- Product Citations
- publications
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hMMP-9 (~93 KD) was activated by 1 mM of p-Aminophenylmercuric acetate (APMA) at different time points at 37°C. After 24h of activation, the mature form hMMP-9 (~60 kD) could be readily observed. Samples at reducing condition were resolved in a SDS-PAGE. Protein per lane: 2.0 mg. -
The activity of monomeric hMMP-9 was measured with 10 µM of fluorogenic MMP substrate, Mca-PLGL-Dpa-AR-NH2 , in the presence of 2.5, 5.0, 10, 20, 40, and 80 ng of activated hMMP-9. The activity of activated hMMP-9 is >800 pmole/min/µg.
MMP-9 is a member of the matrix metalloprotease family of proteins (MMPs). Members of this family are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. Proteolytic activities of MMPs play an important role in vascular remodeling, cellular migration, and the processing of ECM proteins and adhesion molecules. MMP-9 consists of a prodomain, catalytic domain, hinge region, and a hemopexin domain, and is secreted as an inactive zymogen that becomes activated extracellularly. The most relevant natural activators of proMMP-9 are unknown. MMP-9 activation may be mediated by removal of the prodomain by serine proteases or other MMPs, or it may be a direct response to oxidative stress that disrupts the cysteine switch. MMP-9 is capable of processing cytokines and chemokines. For example, it is reported to release the biologically active form of vascular endothelial growth factor (VEGF), indicating that MMP-9 may play a crucial role in the formation of new blood vessels. MMP-9 participates in the migration of immune cells as demonstrated by MMP-9 knockout mice after antigen challenge. Increased MMP-9 expression and activity has been observed to be related to various cancers, as it may contribute to the release of metastatic cells from the bulk tumor as well as their entrance at the site of metastasis by degrading proteins in ECM and basement membrane.
Product DetailsProduct Details
- Source
- Human MMP-9, amino acids (Ala20-Asp707) (Accession# NP_004985.2), is expressed with a C-terminal 8His tag and a linker sequence in the 293E cell line.
- Molecular Mass
- This 722 amino acid recombinant protein has a predicted molecular mass of approximately 80 kD. The protein migrates at about 92 kD in DTT-reducing conditions and about 92 kD in non-reducing condition by SDS-PAGE.The predicted N-terminal amino acid is Ala.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in TCN (25mM Tris, 300mM NaCl, 5mM CaCl2, pH 7.5)
- Endotoxin Level
- Less than 1.0 EU per µg of protein as determine by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 100 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored at -20°C or -70°C for six months. For maximum results, quick spin vial prior to opening. Avoid repeated freeze/thaw cycles.
- Activity
- Human MMP-9 cleaves the peptide substrate Mca-PLGL-Dpa-AR-NH2 with an activity above 800 pmol/min/µg.
- Application
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Bioassay
- Application Notes
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This protein is in the latent form and needs to be activated for bioassay.
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com. - Product Citations
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Antigen Details
- Structure
- Monomer
- Distribution
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MMP-9 is expressed by alveolar macrophages, granulocytes, fibroblasts, and fibrosarcoma cells.
- Function
- Degradation of ECM and connective tissue proteins. Angiogenesis, tissue remodeling, and cancer metastasis. Regulates chemokine activity. Inhibited by TIMPs and α2-macroglobulin (inhibitor of MMPs in bodily fluids).
- Interaction
- ECM proteins
- Ligand/Receptor
- TIMPs
- Bioactivity
- Human MMP-9 cleaves a peptide substrate Mca-PLGL-Dpa-AR-NH2.
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Enzymes and Regulators
- Antigen References
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1. Chakrabarti S and Patel KD. 2005. Exp. Lung Res. 31:599.
2. Klein T and Bischoff R. 2011. Amino Acids 41:271.
3. Raffetto JD and Khalil RA. 2008. Biochem. Pharmacol. 75:346.
4. Bourboulia D and Stetler-Stevenson WG. 2010. Semin. Cancer Biol. 20:161.
5. Vilen ST, et al. 2013. Scientific World Journal. 920595. - Gene ID
- 4318 View all products for this Gene ID
- UniProt
- View information about MMP-9 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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