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- Regulatory Status
- RUO
- Other Names
- Platelet Derived Growth Factor Receptor Beta, Beta Platelet-Derived Growth Factor Receptor, PDGFR-B, PDGFR β, CD140B, CD140b, CD140b Antigen, PDGFR1, PDGF-R-B
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human PDGFRβ inhibits the proliferation of NIH 3T3 mouse fibroblasts induced by human PDGF-BB. -
Stability testing for human PDGF-BB. Human PDGFR b was aliquoted in PBS, pH 7 at 0.1 mg/ml. The aliquots were treated as follows: at 4°C (control), room temperature, and 37°C for one week, and one aliquot was freeze/thawed four times (4x free/thaws). After this procedure, the samples were tested for their ability to inhibit the proliferation of NHI3T3 cells induced by mouse PDGF-BB.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 767204 | 25 µg | 305 CHF | ||||
| 767206 | 100 µg | 867 CHF | ||||
PDGF family includes five members, PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC, and PDGF-DD. Two PDGF receptors (a and b) have been described; they belong to the receptor-tyrosine kinase family, Type III group, which also includes c-KIT, FLT3, and M-CSFR. The two PDGF receptor subtypes show different affinities for the dimeric PDGF isoforms. PDGFRa binds to all ligands except PDGF-D, while PDGFRb binds to PDGF-B and -D only. The two PDGF receptors contain five extracellular immunoglobulin-like domains, a single transmembrane domain, and an intracellular split kinase domain, which is formed by two lobes connected by a flexible polypeptide linker, the kinase insert. The binding of PDGFs to their receptors induce receptor dimerization. PDGF receptors are degraded after activation by a mechanism that involves ligand-induced endocytosis and degradation in lysosomes. Soluble form of PDGFRa was detected in conditioned medium of human cell lines. The soluble receptor was derived by proteolytic clipping of the full length PDGFR. Soluble PDGFRb inhibits hepatic stellate cell activation and attenuates liver fibrosis. Also, soluble PDGFRb inhibits intraosseous growth of breast cancer cells in nude mice. PDGFRb mediates recruitment of pericytes and vascular smooth muscle cells to newly formed vessels in response to PDGF-BB secreted by endothelial cells. PDGFRb knockout embryos develop abnormal glomeruli in the kidney (defect development of mesangial cells) capillary microaneurysm, cardiac and placental defects, and die at E16–E19 from hemorrhage. High levels of PDGFRb expression have been associated with tumor recurrence in primary colorectal cancer, and it correlates with poor disease-free survival in pancreatic, colon, and ovarian cancer patients.
Product DetailsProduct Details
- Source
- Human PDGFRβ, amino acids (Leu33-Phe530) (Accession #NP_002600) was expressed in CHO cells. The carboxy terminus contains SRIEGRMD-IgG-Fc-6His tag.
- Molecular Mass
- The 743 amino acid recombinant protein has a predicted molecular mass of approximately 83.8 kD. The DTT-reduced and non-reduced protein migrate at approximately 150 kD and > 200 kD by SDS-PAGE respectively. The predicted N-terminal amino acid is Leu.
- Purity
- > 95% by SDS-PAGE gel as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS pH 7.2.
- Endotoxin Level
- Less than 1.0 EU per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Human PDGFRβ inhibits the proliferation of NIH 3T3 mouse fibroblasts induced by human PDGF-BB (50 ng/mL) in a dose dependent manner. The ED50 = 0.1 - 0.5 µg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Homodimer
- Distribution
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Cells of mesenchymal origin, mesothelioma cell lines, pericytes and vascular smooth muscle cells.
- Function
- Proliferation, transformation, migration, survival, and apoptosis of mesenchymal cells, in development and pathogenesis. Development of hematopoietic/endothelial precursors, regulates craniofacial development.
- Ligand/Receptor
- PDGF-BB, PDGF -DD
- Bioactivity
- Human PDGFRβ inhibits the activity of human PDGF-BB.
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Cytokine/Chemokine Receptors, Soluble Receptors
- Antigen References
-
1. Tiesman JS, Hart CE. 1993. J. Biol. Chem. 268: 9621.
2. Lindahl P, et al. 1997. Science 277:242.
3. Hart CE, et al. 1998. Science. 240:1529.
4. Borkham-Kamphorst E, et al. 2004 Lab Invest. 84:766.
5. Lennartsson J, et al. 2006. J. Biol. Chem. 281:39152.
6. Van den Akker NM, et al. 2008 Dev. Dyn. 237: 494.
7. Toffalini F, Demoulin JB. 2010. Blood 116:2429.
8. Shan H, et al. 2011. Cancer Sci. 102:1904.
9. Demoulin JB, Montano-Almendras CP. 2012. Am. J. Blood Res. 2:44.
10. Heldin CH, Lennartsson J. 2013. Cold Spring Harb. Perspect. Biol. 5:a009100.
11. Weissmueller S, et al. 2014 Cell. 157:382.
12. Fantauzzo KA, Soriano P. 2016. Genes Dev. 30:2443. - Gene ID
- 5159 View all products for this Gene ID
- UniProt
- View information about PDGFRbeta on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
-
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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