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- Regulatory Status
- RUO
- Other Names
- Stem cell factor, KIT-ligand, Kitl, KL-1, mast cell grow factor (MGF), steel factor (SF), FPH2, SHEP7
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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TF-1 cell proliferation induced by human SCF.
Stem Cell Factor (SCF) is initially synthesized as membrane-bound forms of 248 or 220 amino acids, depending on alternative splicing of exon 6. The 248 amino acid form contains a proteolitic cleveage site encoded by exon 6, and it is cleaved from the cell to release an active soluble protein of 165 amino acid residues. Soluble SCF is glycosylated at both N-linked and O-linked sites. MMP-9 plays a physiological role in SCF release from the membrane, and this action plays a significant role in differentiating and mobilizing stem and progenitors cells from the bone marrow. SCF increases the proliferation of myeloid and lymphoid hematopoietic progenitors in bone marrow cultures. SCF/c-kit interaction in mast cells results in mast cell degranulation with release of mediators, such as histamine and inflammatory cytokines and chemokines. Also, activation of c-kit in dendritic cells regulates T helper cell differentiation and allergic astma. In addition, SCF plays an important role in revascularization of ischemic limbs. Ischemia induces plasma elevation of SCF and thrombopoietin (TPO) and, in lower levels, GM-CSF and erythropoietin (EPO). SCF and TPO induce the release of CXCL12 from platelets, thereby increasing CXCL12 levels in plasma. This results in an extensive mobilization of CXCR4+VEGFR1+ cells (hemangiocytes), accelerating revascularization of the ischemic limbs. SCF binds to its receptor kit that belongs to the type III tyrosine kinase family, whose members include receptors for M-CSF and PDGF.
Product DetailsProduct Details
- Source
- Human SCF, amino acids Glu26-Ala189 (Accession# NM_000899.4) was expressed in E. coli.
- Molecular Mass
- The 165 amino acid recombinant protein has a predicted molecular mass of approximately 18.6 kD. The DTT-reduced and non-reduced protein migrate at approximately 20 kD and 18 kD respectively by SDS-PAGE. The N-terminal amino acid is Met.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
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ED50 =3 -12 ng/ml, corresponding to a specific activity of 0.8 - 3.3 x 105 units/mg, as determined by TF-1 cell proliferation induced in a dose dependent manner.
The specific activity of recombinant human SCF is approximately 1.13 x 103 IU/µg when compared against the WHO Reference Reagent for Human SCF (NIBSC code: 91/682) as determined by the dose dependent stimulation of TF-1 cell proliferation.
For more information on specific activity, please visit the Recombinant Protein Unit Conversions page. - Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Homodimer
- Interaction
- Mast cells, natural killer cells, dendritic cells, eosinophils, epithelial cells, endothelial cells, melanocytes, germ cells, cholangiocytes, platelets, myeloid leukaemia cells, and intestinal cells of Cajal.
- Ligand/Receptor
- c-kit (CD117)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Signal Transduction, Stem Cells
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
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1. Lu HS, et al. 1996. J. Biol. Chem. 271:11309.
2. Heissig B, et al. 2002. Cell 109:625.
3. Jin DK, et al. 2006. Nat. Med. 12:557.
4. Krishnamoorthy N, et al. 2008. Nat. Med. 14:565.
5. Ray P, et al. 2010. Ann. N. Y. Acad. Sci. 1183:104. - Gene ID
- 4254 View all products for this Gene ID
- UniProt
- View information about SCF on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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