Recombinant Human Serpin A12 (Vaspin) (carrier-free)

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Regulatory Status
RUO
Other Names
Vaspin
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Product Citations
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SerpinA12_CF_Human_RECOM_BA_042516
The activity of human Serpin A12 was measured by its ability to inhibit human KLK7. The active human KLK7 was incubated with various concentrations of human Serpin A12 for one hour at room temperature. The remaining activity of human KLK7 was monitored by detecting fluorogenic FRET peptide substrate (Mca-RPKPVE-Nval-WRK(Dnp)-NH2) cleavage. The fluorescence of the product was read at 320/405 nm (Excitation/Emission) in a kinetics mode for five minutes.
  • SerpinA12_CF_Human_RECOM_BA_042516
    The activity of human Serpin A12 was measured by its ability to inhibit human KLK7. The active human KLK7 was incubated with various concentrations of human Serpin A12 for one hour at room temperature. The remaining activity of human KLK7 was monitored by detecting fluorogenic FRET peptide substrate (Mca-RPKPVE-Nval-WRK(Dnp)-NH2) cleavage. The fluorescence of the product was read at 320/405 nm (Excitation/Emission) in a kinetics mode for five minutes.
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756802 10 µg 124 CHF
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756806 100 µg 686 CHF
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Description

Human Serpin A12, also known as Vaspin, is a secreted adipokine that is found in visceral adipose tissue. It belongs to the serpin superfamily, class A. It's known protease target is KLK7, a member of the kallikrein family. In humans, Serpin A12 expression may increase at the onset of diabetes and decrease when the disease aggravates. The administration of recombinant Serpin A12 in a diet-induced mouse obesity model suggests that it may represent an insulin-sensitizing adipokine. Low serum concentration of Serpin A12 may be a risk factor for the progression of type-2 diabetes mellitus. Although its mechanism of action is not fully understood, KLK7 inhibition seems to be the most likely underlying physiological mechanism for the compensatory effects on obesity-induced insulin resistance. In addition to its relation to diabetes, Serpin A12 exerts protective effects on heart disease. Its low concentrations correlate with coronary artery diseases’ severity and unstable angina pectoris. Serpin A12 may inhibit endothelial cell apoptosis, attenuate high glucose stimulated vascular smooth muscle cell proliferation, and chemokinesis. It also acts as a ligand for the cell surface GRP78/voltage dependent anion channel complex in endothelial cells and promotes proliferation, inhibits apoptosis, and protects against diabetes mellitus associated vascular injuries.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human Serpin A12 (Vaspin), amino acids (Leu20-Lys414) (Accession# R78211), was expressed in 293E cells with a C-terminal His-tag.
Molecular Mass
The 410 amino acid recombinant protein has a predicted molecular mass of approximately 47 kD. The protein migrates at approximately 60 kD and 65 kD in both DTT-reducing and non-reducing conditions by SDS-PAGE respectively.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 20 mM Tris, 0.3 M NaCl, pH 7.5.
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Human Serpin A12 (Vaspin) inhibits the peptidase activity of human KLK7 with an IC50 value lower than 60 nM.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer.
Distribution

Expressed in visceral adipose tissues. Possibly present in adipocytes. Present in the plasma and serum.

Function
Adipokines that modulate insulin action by specifically inhibiting target protease KLK7 in white adipose tissues.
Ligand/Receptor
KLK7, GRP78/VDAC complex.
Bioactivity
Human Serpin A12 (Vaspin) activity is measured by its ability to inhibit the peptidase activity of human KLK7.
Biology Area
Neuroscience, Stem Cells
Molecular Family
Enzymes and Regulators
Antigen References

1. Li HL, et al. 2011. Clin. Chem. Lab Med. 49:1547.
2. Jung CH, et al. 2011. Biochem. Biophys. Res. Commun. 413:264.
3. Li H, et al. 2013. Atherosclerosis 228:61.
4. Nakatsuka A, et al. 2012. Diabetes 61:2823.
5. Heiker JT, et al. 2013. Cell Mol. Life Sci. 70:2569.
6. Hida K, et al. 2005. Proc. Natl. Acad. Sci. USA 30:10610.
7. Jian W, et al. 2014. PLoS One 9:e94763.
 

Gene ID
145264 View all products for this Gene ID
UniProt
View information about Serpin A12 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 04.28.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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