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- Regulatory Status
- RUO
- Other Names
- CD172g, SIRPB2, SIRP-B2, bA77C3.1, SIRPgamma
- Ave. Rating
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- Product Citations
- publications
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Recombinant human SIRPG binds to human CD47-Fc Chimera in a dose-dependent manner. EC50 of this effect is 0.15 - 0.9 µg/mL. -
Stability testing for recombinant human SIRPG: Recombinant human SIRPG was aliquoted in PBS, pH 7.2 containing 10% glycerol, at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to bind to human CD47-Fc Chimera in a dose-dependent manner. The EC50 for this effect is 0.15 - 0.9 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 596904 | 25 µg | 380 CHF | ||||
| 596906 | 100 µg | 675 CHF | ||||
The signal regulatory protein (SIRP, CD172, and SHPS) family members are receptor-type I transmembrane glycoproteins known to be involved in negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPs are expressed mainly by immune myeloid cells. Extracellular regions of SIRPs are typically composed of a single V-set and two C1-set immunoglobulin superfamily (IgSF) domains, which, when compared with other members of the IgSF, display a particularly close structural relationship to rearranged antigen receptors (T-Cell receptor and B-Cell receptor). In humans, there are 3 identified SIRP genes clustered on chromosome 20p13; SIRPα, SIRPβ1, and SIRPγ. SIRPγ or SIRPG (signal regulatory protein gamma), has the same ligand i.e., CD47 as SIRPα, are expressed on T cells and activated NK cells and have been proposed to modulate activation of T and NK cells. SIRPG-CD47 interaction mediates cell-cell adhesion and supports APC-T cell contact, enhancing antigen presentation, resulting in T cell proliferation and cytokine secretion. It seems unlikely that SIRPG itself generates intracellular signals because it does not have any recognizable signaling motifs.
Product DetailsProduct Details
- Source
- Human SIRPG, amino acids Glu29-Pro360 (Accession # Q9P1W8), with a C-terminal 6x His tag, was expressed in CHO cells.
- Molecular Mass
- The 338 amino acid recombinant protein has a predicted molecular mass of approximately 37.6 kD. The DTT-reduced and non-reduced protein migrate at approximately 40 - 50 kD in SDS-PAGE.
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N-terminal
Sequence Analysis - The predicted N-terminal amino acid is Glu.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2 with 10% glycerol
- Endotoxin Level
- Less than 0.1 EU per μg of cytokine as determined by the LAL method
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human SIRPG binds to human CD47-Fc Chimera in a dose-dependent manner. EC50 of this effect is 0.15 - 0.9 µg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
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CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells
- Function
- T cell and NK cell activation, cell-cell adhesion, supports APC-T cell contact, enhance antigen presentation, T cell proliferation and cytokine secretion
- Interaction
- CD47 expressing cells
- Ligand/Receptor
- CD47
- Bioactivity
- Recombinant human SIRPG binds to human CD47-Fc Chimera in a dose-dependent manner. EC50 of this effect is 0.15 - 0.9 µg/mL.
- Cell Type
- Antigen-presenting cells, Granulocytes, Monocytes
- Molecular Family
- CD Molecules
- Antigen References
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- Ichigotani Y, et al. 2000. J Hum Genet. 45:378-82.
- Nettleship JE, et al. 2013. Crystal BMC Struct. Biol. 13:13.
- Sinha S, et al. 2018. Sci Rep. 8:15440.
- Sinha S, et al. 2019. Clin Immunol. 198:57.
- Sharp RC, et al. 2021. Front Immunol. 12:739048.
- Brooke G, et al. 2004. J Immunol. 173:2562-70.
- Piccio L, et al. 2005. Blood. 105:2421-7.
- Van Beek EM, et al. 2005. J Immunol. 175:7781-7.
- Gene ID
- 55423 View all products for this Gene ID
- UniProt
- View information about SIRPG on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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