Recombinant Human TNFSF18 (GITRL) (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
TL6, AITRL, GITRL, GITR Ligand, Tumor necrosis factor superfamily, Member 18
Ave. Rating
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Product Citations
publications
Human_TNFSF18_CF_RECOM_040819_updated.png
Human TNFSF18 (GITRL) binds to immobilized recombinant human TNFRSF18 (GITR) (Cat. No. 764302) in a dose-dependent manner. The ED50 for this effect is 5 – 20 ng/mL
  • Human_TNFSF18_CF_RECOM_040819_updated.png
    Human TNFSF18 (GITRL) binds to immobilized recombinant human TNFRSF18 (GITR) (Cat. No. 764302) in a dose-dependent manner. The ED50 for this effect is 5 – 20 ng/mL
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559202 10 µg 147 CHF
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559204 25 µg 276 CHF
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559206 100 µg 879 CHF
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Description

TNFSF18 is a type II transmembrane glycoprotein that belongs to the TNF superfamily. This protein is mainly expressed on antigen presenting cells (APC) including macrophages, B cells, dendritic cells (DCs), and endothelial cells. Its expression increases upon the stimulation of proinflammatory stimuli. The receptor of TNFSF18 is the glucocorticoid-induced TNFR-related protein (GITR), which is a member of the TNFR superfamily. Engagement of TNFSF18 and GITR leads to T cell activation, proliferation, and cytokine production. It also inhibits the suppressive function of T regulatory cells and induces macrophage activation. GITR-TNFSF18 interaction plays an essential role in the regulation of the immune response to self antigens and pathogens. In addition, this receptor-ligand interaction has been shown to modulate anti-tumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human TNFSF18, amino acids (Glu74-Ser199) (Accession# NP_005083), was expressed in 293E cells with an N-terminal His tag.
Molecular Mass
The 147 amino acid recombinant protein has a predicted molecular mass of approximately 16.5 kD. The protein migrates at about 25 kD in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is His.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 1.0 EU per µg of protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Human TNFSF18 (GITRL) binds to immobilized recombinant human TNFRSF18 (GITR) (Cat. No. 764302) in a dose-dependent manner. The ED50 for this effect is 5 – 20 ng/mL
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Ming S, et al. 2021. Front Immunol. 12:736269. PubMed

Antigen Details

Structure
Monomer.
Distribution

Macrophages, B cells, dendritic cells (DCs), and endothelial cells.

Function
TNFSF18 plays roles in regulating T cell activation and natural killer cell anti-tumor activity. It is induced by inflammatory processes.
Interaction
T cells.
Ligand/Receptor
GITR.
Bioactivity
TNFSF18, when immobilized with anti-CD3 antibody, is able to inhibit the secretion of IFN-γ by activated T cells.
Cell Type
Embryonic Stem Cells
Biology Area
Cell Proliferation and Viability, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433.
2. Kim JD, et al. 2003. Genes Immun. 4:564.
3. Azuma M. 2010. Crit. Rev. Immunol. 30:547.
4. Hanabuchi S, et al. 2006. Blood 107:3617.
5. Muriglan SJ, et al. 2004. J. Exp. Med. 200:149.
6. Shevach EM and Stephens GL. 2006. Nat. Rev. Immunol. 6:613.
7. Baltz K, et al. 2007. FASEB J. 21:2442.
8. Placke T, et al. 2010. Clin. Dev. Immunol. 2010:239083.

Gene ID
8995 View all products for this Gene ID
UniProt
View information about TNFSF18 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 2    Revision Date: 04.08.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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