Recombinant Human VEGF-C (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Vascular endothelial growth factor related protein (VRP), Flt4 ligand, Flt4-L
Ave. Rating
Submit a Review
Product Citations
publications
VEGF-C_Human_Recombinant_Protein_BA_062513
HUVEC cell proliferation induced by human VEGF-C.
  • VEGF-C_Human_Recombinant_Protein_BA_062513
    HUVEC cell proliferation induced by human VEGF-C.
Cat # Size Price Quantity Check Availability Save
589702 10 µg 147 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
589704 25 µg 253 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
589706 100 µg 686 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote

Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

VEGF-C was initially cloned from human prostatic adenocarcinoma cell line PC-3 and human glioma G61 cell cDNA libraries. VEGF-C belongs to the VEGF family that includes the following members, VEGF-A, VEGF-B, VEGF-C, VEGF-D, and PlGF (placental growth factor). In addition, viral VEGF homologs (collectively called VEGF-E) and snake venom VEGFs such as T.f. (Trimeresurus flavoviridis) and svVEGF (called VEGF-F) have been described. The homologus portions of VEGF-C are ~30% identical to VEGF165, ~27% to VEGF-B167, ~25% to PlGF- 1, and ~22-24% to PDGF-A and PDGF-B. VEGF-C possesses a C-terminal silk-protein-like structural motif (pattern of spacing cysteine residues) not present in other member of the VEGF family. VEGF-C binds to VEGFR3 that is almost exclusively expressed in lymphatic endothelium in adults, whereas in embryos (mid-gestation) it is expressed on blood vessels and is required for blood vascular remodeling. VEGFR3 is shared by VEGF-C and VEGF-D. VEGF-C is required for sprouting of the first lymphatic vessels from embryonic veins. Also, VEGF-C promotes survival of retinal vascular endothelial cells via VEGFR2. High levels of VEGF-C (in serum) have been associated to dyslipidemia and atherosclerosis. In addition, elevated levels of VEGF-C are increased in the serum of patients with some cancers (primary nonsmall cell lung carcinoma, cervical carcinoma, and gastric cancer among others) and it has been suggested that it is correlated with lymph node metastases and poor prognosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human VEGF-C, amino acids (Thr103-Arg227) (Accession# NM_005429.2) was expressed in CHO cells. The recombinant has a C-terminal 10His tag.
Molecular Mass
The 135 amino acid recombinant protein has a predicted molecular mass of approximately 15.4 kD. The DTT-reduced and non-reduced protein migrate at approximately 23-28 and 20-25 kD by SDS-PAGE respectively. The N-terminal amino acid is Thr.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 100 - 500 ng/ml, corresponding to a specific activity of 0.2 - 1.0 x 104 units/mg, as determined by the dose dependent stimulation of HUVEC cell proliferation.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Distribution
VEGF-C messenger RNA is expressed in multiple tissues such as heart, placenta, muscle, ovary, and small intestine. VEGF-C is expressed by endothelial cells.
Function
VEGF-C plays a key role in lymphangiogenesis, stimulates the migration, proliferation, and survival of human endothelial cells. Survivin regulates expression of VEGF-C in breast cancer cells. VEGF-C is induced by IL-1 and TNF in endothelial cells.
Interaction
Lymphatic endothelial cells, endothelial cells, and subsets of leukemic cells.
Ligand/Receptor
VEGFR3 (Flt4), VEGFR2 (KDR/FLK1), VEGFR2/VEGFR3 heterodimer.
Cell Type
Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology, Angiogenesis
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Joukov V, et al. 1996. J. EMBO. 15:290.
2. Dias S, et al. 2002. Blood 99:2179.
3. Karkkainen MJ, et al. 2004. Nat. Immunol. 5:74.
4. Nilsson I, et al. 2010. J. EMBO. 20:1377.
5. Tanaka T, et al. 2010. J. Exp. Clin. Can. Res. 29:83.
6. Wada H, et al. 2011. PlosOne 6:e29351
7. Cai X, et al. 2012. Diagn. Pathol. 7:52.

Gene ID
7424 View all products for this Gene ID
UniProt
View information about VEGF-C on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 06.25.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account