Recombinant Mouse IL-36γ (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin 1 family, member 9 (IL-1F9), IL-1F, IL-1ε, IL-1H1
Ave. Rating
Submit a Review
Product Citations
publications
IL36g_Mouse_RECOM_BA_020923
Mouse IL-36γ induces IL-6 secretion in 3T3L1 preadipocytes, in a dose-dependent manner. BioLegend’s Mouse IL-36γ product was compared side-by-side to the leading competitor’s equivalent product.
  • IL36g_Mouse_RECOM_BA_020923
    Mouse IL-36γ induces IL-6 secretion in 3T3L1 preadipocytes, in a dose-dependent manner. BioLegend’s Mouse IL-36γ product was compared side-by-side to the leading competitor’s equivalent product.
Cat # Size Price Quantity Check Availability Save
552806 100 µg 1167 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
552802 10 µg 223 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
552804 25 µg 440 CHF
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The IL-1 family is a group of cytokines comprised of 11 members, including the recently renamed IL-36 cytokines, IL-36α, β, and γ (previously known as IL-1F6, IL-1F8, and IL-1F9, respectively). IL-36γ signals through the IL-1 receptor family members IL-1Rrp2 and IL-1RAcP and can activate downstream MAP kinases and transcription factor NF-κB. IL-36γ is synthesized without signal peptide and the secretion pathway is unclear. It has been shown that like other IL-36 cytokines, the N-terminal processing of IL-36γ can significantly increase its biological activity. The possible processing enzymes are currently unknown. IL-36γ plays a significant role in the immune response, as low levels of IL-36 receptor IL-1Rrp can be detected on bone marrow-derived dendritic cells and CD4+ T cells. IL-36γ treatment can upregulate CD80, CD86 and MHC Class II, and proinflammatory cytokine production in dendritic cells. IL-36γ has also been implicated in airway hyperesponsiveness. Intranasal administration of recombinant IL-36γ in mice leads to epithelial cell hypertrophy, cellular infiltration, and mucus production. IL-36γ expression in the lungs of mice can be upregulated by allergic inflammation. In the skin, IL-36γ is primarily expressed by keratinocytes and its expression can be upregulated by inflammatory cytokines, toll-like receptor ligands, and some pathological conditions. IL-36γ is highly induced in psoriasis plaques.    

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse IL-36γ, amino acids Gly13-Ser164 (Accession# Q8R460), was expressed in E. coli.
Molecular Mass
The 152 amino acid recombinant protein has a predicted molecular mass of approximately 20 kD. The DTT-reduced and non-reduced protein migrates at approximately 20 kD by SDS-PAGE. The predicted N-terminal amino acid is Gly.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 20 mM MOPS, pH 7.4, 150 mM NaCl, 10 mM TCEP.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The ED50 is 1 - 5 ng/ml, corresponding to a specific activity of 0.2 - 1.0 x 106 units/mg, as determined by a dose-dependent stimulation of IL-6 secretion in 3T3L1 preadipocytes.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Kluwig D, et al. 2021. Life (Basel). 11:. PubMed

Antigen Details

Structure
IL-1 family cytokine.
Distribution

Peripheral blood lymphocytes, keratinocytes, bronchial epithelial cells.

Function
IL-36γ is a proinflammatory cytokine. It is upregulated by inflammatory cytokines, toll-like receptor ligands, and some pathological conditions in skin. Truncation of IL-36α, IL-36β, and IL-36γ enhances their activity.
Interaction
Dendritic cells, macrophages, lung fibroblasts.
Ligand/Receptor
A combination of IL-1Rrp2 and IL-1RAcP.
Biology Area
Angiogenesis, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Blumberg H, et al. 2007. J. Exp. Med. 204:2603.
2. Vigne S, et al. 2011. Blood. 118: 5813.
3. Ramadas RA, et al. 2006. Immunogenetics. 58:851.
4. Carrier Y, et al. 2011. J. Invest. Dermatol. 131:2428.
5. Debets R, et al. 2001. J. Immunol. 167:1440.
6. Towne J, et al. 2004. J. Biol. Chem. 279:13677.
7. Towne J, et al. 2011. J. Biol. Chem. 286:42594.

Gene ID
215257 View all products for this Gene ID
UniProt
View information about IL-36gamma on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 4    Revision Date: 09.22.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account