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Recombinant Mouse Latent TGF-β (ELISA Std.)

Pricing & Availability
Regulatory Status
RUO
Other Names
Latent TGF-β1, Latent TGFβ, Latent TGFβ1, LTGF-β1, LTGF-β, LTGFβ1, LTGFβ, Latent transforming growth factor beta
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Product Citations
publications
Latent_TGFb_Mouse_ELISA_020514
  • Latent_TGFb_Mouse_ELISA_020514
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594509 4 pack 94 CHF
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Description

Latent TGF-β is secreted as a complex consisting of LAP and TGF-β1. LAP and TGF-β1 are synthesized as a single propeptide precursor of 390 amino acids with an N-terminal signal peptide of 29 amino acids, a 249 amino acids pro-region (LAP), and a 112 amino acids C-terminal region (TGF-β1).

Both LAP and TGF-β1 exist as homodimers in circulation, but the disulfide linked homodimers of LAP and TGF-β1 remain non-covalently associated, forming the small latent TGF-β complex (SLC, 100 kD). The large latent TGF-β complex (LLC, 235-260 kD) contains a third component, the latent TGF-β binding protein (LTBP), which is linked to LAP by a single disulfide bond. LTBP does not confer latency but is for efficient secretion of the complex to extracellular sites.

TGF-β1 and LAP can be released (activated) by many factors, including enzymes and low or high pH. TGF-β1 and LAP have multiple functions, such as regulating proliferation and differentiation of various cell types.

Increased production and activation of latent TGF-β have been linked to immune defects associated with malignancy and autoimmune disorders, to susceptibility to opportunistic infection, and to the fibrotic complications associated with chronic inflammatory conditions.

Latent TGF-β has been found to protect against glomerulonephritis. It is a surface marker of activated regulatory T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Full-length mouse TGF-β1 was expressed in P3U1 cells, and naturally processed by furin cleavage to produce latent TGF-β.
Molecular Mass
The predicted molecular mass of mouse latent TGF-β is ~100 kD.
Purity
The recombinant protein is unpurified transfected cell culture supernatant.
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application

ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay.  A standard curve comprised of two-fold dilutions from 0.06 ng/ml to 4 ng/ml is recommended, and the end-user should titrate each lot of reagents for optimal performance.

Application Notes

To measure mouse latent TGF-ß, this recombinant protein can be used as a standard in sandwich ELISA format, when used in conjunction with the LEAF™ purified TW7-7H4 antibody (Cat. No. 361201) as the capture antibody, and the biotinylated TW7-16B4 antibody (Cat. No. 141411) as the detection antibody.

Note: For testing mouse latent TGF-ß in serum, plasma or supernatant, LEGEND MAX™ Mouse Latent TGF-ß ELISA Kit (Cat. No. 433007 & 433008) is specially developed and recommended.

Product Citations
  1. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed

Antigen Details

Structure
Homodimer
Distribution

Latent TGF-β is secreted by numerous cells, and activated Treg cells.

Function
Latent TGF-β is the inactive form of TGF-β1 and LAP.
Ligand/Receptor
Latent TGF-β binding proteins (LTBPs).
Cell Type
Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Lu M, et al. 2002. J. Cell Sci. 115:4641.
2. Khalil N. 1999. Microbes Infect. 1:1255.
3. Probst-Kepper M, and Buer J. 2010. Biol. Direct 5:8.
4. Ali N, et al. 2008. PLoS One 3:e1914.

Gene ID
21803 View all products for this Gene ID
UniProt
View information about TGF-beta1 on UniProt.org
Go To Top Version: 3    Revision Date: 09.29.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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