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- Regulatory Status
- RUO
- Other Names
- TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor,Transforming growth factor-beta receptor type II, Tgfbr2
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Recombinant mouse TGFBR2-Fc Chimera inhibits TGF-β1 activity on HT-2 (mouse T cells) in presence of 10 ng/mL mouse IL-4. -
Immobilized recombinant human TGF-β1 binds to mouse TGFBR2-Fc Chimera in a dose-dependent manner. The ED50 for this effect is 8-48 ng/mL. -
Stability testing for recombinant mouse TGFBR2-Fc Chimera: Recombinant mouse TGFBR2-Fc Chimera was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control) for 7 days and another aliquot was frozen and thawed four times (4x Freeze/Thaw) over a period of one week. After this procedure, samples were tested for their ability to bind to recombinant human TGF-β1 in a dose-dependent manner (C). The ED50 for this effect is 8-48 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 718654 | 25 µg | 385 CHF | ||||
| 718656 | 100 µg | 670 CHF | ||||
Transforming growth factor β (TGF-β) family members signal via hetero-tetrameric complexes of type I and type II dual specificity kinase receptors. The activation and stability of the receptors are controlled by post-translational modifications, such as phosphorylation, ubiquitylation, sumoylation and neddylation. Activation of TGF-β receptors induces signaling via formation of Smad complexes that are translocated to the nucleus where they act as transcription factors. TGF-β receptors may also signal via non-Smad pathways, including the Erk1/2, JNK and p38 MAP kinase pathways and the Src tyrosine kinase, phosphatidylinositol 3′-kinase, and Rho GTPases. TGF-β1 and TGF-β3 bind TGFBR2 with higher affinity than TGFBR1. Thus, the binding occurs first to TGFBR2; thereafter, TGFBR1 is recruited to the complex by recognizing a unique interface generated by the TGF-β–TGFBR2 complex. The gene-encoding TGFBR2 is frequently mutated (30% of all cases) in colorectal cancer patients. Increased activity of TGF-β signaling is associated with tumor progression, fibrosis, immunosuppression and other diseases, has prompted the development of TGF-β signaling inhibitors. Such inhibitors include inhibitory antibodies against TGF-β or TGF-β ligand traps consisting of soluble extracellular domains of TGF-β receptors. Moreover, an antibody against ALK-1 was found to inhibit endothelial cell sprouting and angiogenesis.
Product DetailsProduct Details
- Source
- Mouse TGFBR2-Fc Chimera, amino acids Ile24-Asp184 (Accession # Q62312), with a C-terminal Fc tag, was expressed in CHO cells.
- Molecular Mass
- The 401 amino acid recombinant protein has a predicted molecular mass of approximately 45.2 kD. The DTT-reduced and non-reduced protein migrate at approximately 60 kD and 115 kD respectively in SDS-PAGE.
-
N-terminal
Sequence Analysis - The predicted N-terminal amino acid is Ile.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per μg of protein as determined by the LAL method
- Concentration
- 25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Mouse TGFBR2-Fc Chimera inhibits TGF-β1 activity on HT-2 (mouse T cells) in presence of 10ng/mL mouse IL-4. Immobilized recombinant human TGF-β1 (Cat. No. 781802) binds to mouse TGFBR2-Fc Chimera in a dose-dependent manner. The ED50 for this effect is 8-48 ng/mL.
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Monomer
- Distribution
-
Expression on most cell types
- Function
- Cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis
- Ligand/Receptor
- Binds with TGF-β1 and TGF-β3 with high affinity and at much lower affinity with TGF-β2
- Bioactivity
- Mouse TGFBR2-Fc Chimera inhibits TGF-β1 activity on HT-2 (mouse T cells) in presence of 10 ng/mL mouse IL-4. Immobilized recombinant human TGF-β1 (Cat. No. 781802) binds to mouse TGFBR2-Fc Chimera in a dose dependent manner. The ED50 for this effect is 8-48 ng/mL.
- Cell Type
- Tfh, Th1, Th17, Th2, Tregs
- Biology Area
- Adaptive Immunity, Cancer Biomarkers, Cell Proliferation and Viability
- Molecular Family
- Fc Receptors, Soluble Receptors
- Antigen References
-
- Carl-Henrik Heldin and Aristidis Moustakas. 2016. Cold Spring Harb Perspect Biol. 8:a022053.
- Rik Derynck and Erine H Budi 2019. Sci Signal .12:5183.
- Akhurst RJ and Hata A 2012. Nat Rev Drug Discov. 11:790-811.
- Zhong ZJ, et al. 2010. Clin Cancer Res. 16:1191-1205.
- Van Meeteren LA, et al. 2012. J Biol Chem. 287:18551-18561.
- Groppe J, et al. 2008. Mol Cell. 29:157-68.
- Huse M, et al. 2001. Mol Cell. 8: 671-682.
- Kavsak P, et al. 2000. Mol Cell. 6: 1365-1375.
- Ebisawa T, et al. 2001. J Biol Chem. 276:12477-12480.
- Biswas S, et al. 2008. Genes Chromos Cancer. 47 95-106.
- Gene ID
- 21813 View all products for this Gene ID
- UniProt
- View information about TGFBR2 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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