- Clone
- 9E2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- NKp46, NCR1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is directly involved in target cell recognition and lysis, and is exclusively expressed on CD3-CD56+ NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- NKp46-Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
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Blue Laser (488 nm)
- Application Notes
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Clone 9E2 has been shown to block NK activation through NKp46.6
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
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Application References
(PubMed link indicates BioLegend citation) - RRID
-
AB_3662557 (BioLegend Cat. No. 285253)
Antigen Details
- Structure
- Type I membrane glycoprotein (46 kD)
- Distribution
-
Expressed on resting and activated NK cells
- Cell Type
- NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656. - Gene ID
- NA
- UniProt
- View information about CD335 on UniProt.org
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD335 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD335 (NKp46)
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Biotin anti-human CD335 (NKp46)
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PE anti-human CD335 (NKp46)
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Alexa Fluor® 647 anti-human CD335 (NKp46)
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Pacific Blue™ anti-human CD335 (NKp46)
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Brilliant Violet 421™ anti-human CD335 (NKp46)
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PerCP/Cyanine5.5 anti-human CD335 (NKp46)
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APC anti-human CD335 (NKp46)
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PE/Cyanine7 anti-human CD335 (NKp46)
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FITC anti-human CD335 (NKp46)
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Brilliant Violet 510™ anti-human CD335 (NKp46)
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Brilliant Violet 605™ anti-human CD335 (NKp46)
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Brilliant Violet 650™ anti-human CD335 (NKp46)
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PE/Dazzle™ 594 anti-human CD335 (NKp46)
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Alexa Fluor® 700 anti-human CD335 (NKp46)
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Brilliant Violet 711™ anti-human CD335 (NKp46)
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APC/Fire™ 750 anti-human CD335 (NKp46)
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Alexa Fluor® 488 anti-human CD335 (NKp46)
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TotalSeq™-B0101 anti-human CD335 (NKp46)
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TotalSeq™-C0101 anti-human CD335 (NKp46)
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TotalSeq™-A0101 anti-human CD335 (NKp46)
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Brilliant Violet 785™ anti-human CD335 (NKp46)
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Ultra-LEAF™ Purified anti-human CD335 (NKp46)
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APC/Cyanine7 anti-human CD335 (NKp46) Antibody
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PE/Cyanine5 anti-human CD335 (NKp46)
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PE/Fire™ 810 anti-human CD335 (NKp46)
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TotalSeq™-D0101 anti-human CD335 (NKp46)
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PE/Cyanine7 anti-human CD335
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Spark Red™ 718 anti-human CD335 (NKp46) (Flexi-Fluor™)
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GMP PE/Cyanine7 anti-human CD335 (NKp46)
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Spark Blue™ 574 anti-human CD335 (NKp46) (Flexi-Fluor™)
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Spark Blue™ 550 anti-human CD335 (NKp46) (Flexi-Fluor™)
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