Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™) Antibody, CD44, IM7

Pricing & Availability

Clone: IM7 (See other available formats)
Regulatory Status: RUO
Other Names: Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype: Rat IgG2b, κ
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Product Citations: publications

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285013

50 µg

336 CHF

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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse, Human
Reported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD44^17,18 that is located between amino acids 145 and 186²⁰. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections^6,7, complement-mediated cytotoxicity¹, immunoprecipitation^1,3, in vivo inhibition of DTH^4,5, and spatial biology (IBEX)^23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3106279 (BioLegend Cat. No. 285013)

Antigen Details

Structure: Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution: All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
Function: Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor: Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type: B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.
Gene ID: 12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt: View information about CD44 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD44 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse/human CD44	IM7	FC
Biotin anti-mouse/human CD44	IM7	FC,ICC,IHC-P
FITC anti-mouse/human CD44	IM7	FC,ICC,IHC-P
PE/Cyanine5 anti-mouse/human CD44	IM7	FC
Purified anti-mouse/human CD44	IM7	FC,IHC-F,CyTOF®,ELISA,ICC,IHC-P,IP,CMCD,Stim
Brilliant Violet 605™ anti-mouse/human CD44	IM7	FC
PE anti-mouse/human CD44	IM7	FC
Alexa Fluor® 488 anti-mouse/human CD44	IM7	FC,ICC,IHC-P,SB
Alexa Fluor® 647 anti-mouse/human CD44	IM7	FC,ICC,SB
Pacific Blue™ anti-mouse/human CD44	IM7	FC
Alexa Fluor® 700 anti-mouse/human CD44	IM7	FC,SB
PE/Cyanine7 anti-mouse/human CD44	IM7	FC
APC/Cyanine7 anti-mouse/human CD44	IM7	FC
PerCP/Cyanine5.5 anti-mouse/human CD44	IM7	FC
PerCP anti-mouse/human CD44	IM7	FC
Brilliant Violet 421™ anti-mouse/human CD44	IM7	FC,ICC,IHC-P
Brilliant Violet 570™ anti-mouse/human CD44	IM7	FC
Brilliant Violet 785™ anti-mouse/human CD44	IM7	FC
Brilliant Violet 510™ anti-mouse/human CD44	IM7	FC
Ultra-LEAF™ Purified anti-mouse/human CD44	IM7	FC,CyTOF®,ELISA,IHC,IP,CMCD,Stim
Brilliant Violet 650™ anti-mouse/human CD44	IM7	FC
Purified anti-mouse/human CD44 (Maxpar® Ready)	IM7	FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD44	IM7	ICC,FC,IHC-P,SB
PE/Dazzle™ 594 anti-mouse/human CD44	IM7	FC
Brilliant Violet 711™ anti-mouse/human CD44	IM7	FC
APC/Fire™ 750 anti-mouse/human CD44	IM7	FC
TotalSeq™-A0073 anti-mouse/human CD44	IM7	PG
TotalSeq™-C0073 anti-mouse/human CD44	IM7	PG
TotalSeq™-B0073 anti-mouse/human CD44	IM7	PG
Spark YG™ 570 anti-mouse/human CD44	IM7	FC,IHC-P
Spark YG™ 593 anti-mouse/human CD44	IM7	FC
TotalSeq™-D0073 anti-mouse/human CD44	IM7	PG
Brilliant Violet 750™ anti-mouse/human CD44	IM7	FC
PerCP/Fire™ 806 anti-mouse/human CD44	IM7	FC
Spark Red™ 718 anti-mouse/human CD44	IM7	FC
PE/Fire™ 810 anti-mouse/human CD44	IM7	FC
Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™)	IM7	FC
Spark Red™ 718 anti-mouse/human CD44 (Flexi-Fluor™)	IM7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02.06.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.