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Spark Blue™ 574 anti-human CD19 Antibody

Pricing & Availability
Clone
SJ25C1 (See other available formats)
Regulatory Status
RUO
Other Names
CD19 Molecule, CD19 Antigen, Differentiation Antigen CD19, B-Lymphocytes Surface Antigen B4, T-cell Surface Antigen Leu-12, CVID3, B4, B-lymphocyte Antigen CD19
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SJ25C1_SparkBlue574_CD19_Antibody_092823
Human peripheral blood lymphocytes were stained with anti-human CD20 Brilliant Violet 421™ and anti-human CD19 (clone SJ25C1) Spark Blue™ 574 (left) or stained with anti-human CD20 Brilliant Violet 421™ only (right).
  • SJ25C1_SparkBlue574_CD19_Antibody_092823
    Human peripheral blood lymphocytes were stained with anti-human CD20 Brilliant Violet 421™ and anti-human CD19 (clone SJ25C1) Spark Blue™ 574 (left) or stained with anti-human CD20 Brilliant Violet 421™ only (right).
Compare all formats See Spark Blue™ 574 spectral data
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363047 25 tests 120 CHF
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363048 100 tests 305 CHF
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NALM1 + NALM16 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 574 has a maximum excitation of 506 nm and a maximum emission of 574 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone SJ25C1 partially blocks anti-human CD19 clones HIB19 and 4G7 staining based on in-house testing

RRID
AB_3083352 (BioLegend Cat. No. 363047)
AB_3083352 (BioLegend Cat. No. 363048)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD.
Distribution

B lineage (except plasma cells), follicular dendritic cells.

Function
B cell activation and differentiation.
Interaction
Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells and interacts with VAV and LYN. It also interacts with GRAB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378 and with PLCG2 when phosphorylated on Tyr-409.
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR co-receptor.
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 1    Revision Date: 09.13.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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