- Clone
- 11C3C65 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD223, LAG-3, LAG3, lymphocyte-activation gene-3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
369355 | 25 tests | 140 CHF | ||||
369356 | 100 tests | 330 CHF |
CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human LAG-3 transfected cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark PLUS B550™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark PLUS B550™ has a maximum excitation of 516 nm and a maximum emission of 540 nm. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.
Antigen Details
- Structure
- 70 kD transmembrane glycoprotein, Ig superfamily, highly homologous to CD4.
- Distribution
-
Activated T-cells and natural killer cells (NKs) and regulatory T cells.
- Function
- Negatively regulates T-cell activation.
- Ligand/Receptor
- Binds MHC class II molecules.
- Cell Type
- Dendritic cells, NK cells, T cells, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Castelli C, et al. 2014. Oncoimmunology. 3(11):e967146.
2. Poirier N, et al. 2011. Clin. Exp. Immunol. 164:265.
3. Juno JA, et al. 2015. Retrovirology. 12:17.
4. Casati C, et al. 2006. Cancer Res. 66:4450. - Gene ID
- 3902 View all products for this Gene ID
- UniProt
- View information about CD223 on UniProt.org
Related FAQs
Other Formats
View All CD223 (LAG-3) Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD223 (LAG-3)
PHA stimulated (3-day) human peripheral blood lymphocytes we... -
Alexa Fluor® 647 anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
PE anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
FITC anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
PE/Cyanine7 anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
PerCP/Cyanine5.5 anti-human CD223 (LAG-3)
Peripheral blood mononuclear cells stimulated with anti-CD3/... -
Brilliant Violet 421™ anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
Brilliant Violet 650™ anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
Brilliant Violet 510™ anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
Brilliant Violet 785™ anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
Brilliant Violet 711™ anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
Brilliant Violet 605™ anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
Alexa Fluor® 488 anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
Biotin anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
PE/Dazzle™ 594 anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
APC/Fire™ 750 anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
TotalSeq™-A0152 anti-human CD223 (LAG-3)
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TotalSeq™-C0152 anti-human CD223 (LAG-3)
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TotalSeq™-B0152 anti-human CD223 (LAG-3)
-
TotalSeq™-D0152 anti-human CD223 (LAG-3)
-
Alexa Fluor® 700 anti-human CD223 (LAG-3)
Peripheral blood mononuclear cells were stimulated with anti... -
Pacific Blue™ anti-human CD223 (LAG-3)
Peripheral blood mononuclear cells stimulated with anti-CD3/... -
PE/Cyanine5 anti-human CD223 (LAG-3)
Anti-CD3/CD28 and recombinant IL-2 stimulated (three days) p... -
APC/Cyanine7 anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (3 days) peripheral blood mononucle... -
APC/Fire™ 810 anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
Brilliant Violet 750™ anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (three days) peripheral blood monon... -
Spark Red™ 718 anti-human CD223 (LAG-3) (Flexi-Fluor™)
-
Spark PLUS UV395™ anti-human CD223 (LAG-3)
CD3/CD28/IL-2 stimulated (3 days) human peripheral blood mon... Unstimulated peripheral blood mononuclear cells were stained... -
Spark Blue™ 574 anti-human CD223 (LAG-3) (Flexi-Fluor™)
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Spark PLUS B550™ anti-human CD223 (LAG-3) Antibody
CD3/CD28/IL-2 stimulated (3 days) human peripheral blood mon... Unstimulated human peripheral blood mononuclear cells were s...
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