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Spark PLUS B550™ anti-human CD366 (Tim-3) Antibody

Pricing & Availability
Clone
A18087E (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Mouse IgG1, κ
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Product Citations
publications
A18087E_Spark-PLUS-B550_CD366_FC_082824
PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with anti-human CD4 (clone SK3) Spark YG™ 581 and anti-human CD366 (Tim-3) (clone A18087E) Spark PLUS B550™ (left) or stained with anti-human CD4 (clone SK3) Spark YG™ 581 only (right).
  • A18087E_Spark-PLUS-B550_CD366_FC_082824
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with anti-human CD4 (clone SK3) Spark YG™ 581 and anti-human CD366 (Tim-3) (clone A18087E) Spark PLUS B550™ (left) or stained with anti-human CD4 (clone SK3) Spark YG™ 581 only (right).
Compare all formats See Spark PLUS B550™ spectral data
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364813 25 tests 140 CHF
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Tim-3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark PLUS B550™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark PLUS B550™ has a maximum excitation of 516 nm and a maximum emission of 540 nm.

Excitation Laser
Blue Laser (488 nm)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells

Function
CD366 plays a role in regulating macrophage activation, checkpoint inhibitor, T cell apoptosis and immune tolerance
Ligand/Receptor
Galectin-9 and PtdSer
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Hafler DA and Kuchroo V. 2008. J Exp Med. 205:2699.
  2. Zhu C, et al. 2005. Nat Immunol. 6:1245.
  3. Wang F, et al. 2009. Immunobiology. 214:342.
Gene ID
84868 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org
Go To Top Version: 1    Revision Date: 08.28.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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