Spark PLUS UV395™ anti-human IFN-γ Antibody, IFN-γ, 4S.B3

Pricing & Availability

Clone: 4S.B3 (See other available formats)
Regulatory Status: RUO
Other Names: Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype: Mouse IgG1, κ
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Product Citations: publications

4SB3_SparkPLUS395_IFN-y_Antibody_1_100824 — Human peripheral blood lymphocytes were stimulated with PMA/Ionomycin for 6 hours, fixed, permeabilized, and then surface stained with anti-human CD3 (clone UCHT1) APC and intracellularly stained with anti-human IFN-γ (clone 4S.B3) Spark PLUS UV395™

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502559

25 tests

160 CHF

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502560

100 tests

325 CHF

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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partially purified, native human IFN-γ
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark PLUS UV395™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark PLUS UV395™ has a maximum excitation of 355 nm and a maximum emission of 385 nm.
Excitation Laser: Ultraviolet Laser (355 nm)
Application Notes: ELISA or ELISPOT Detection⁵: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.
Flow Cytometry^3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization^1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
RRID: AB_3097565 (BioLegend Cat. No. 502559)
AB_3097565 (BioLegend Cat. No. 502560)

Antigen Details

Structure: Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources: CD8⁺ and CD4⁺ T cells, NK cells
Cell Targets: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.
Regulation: Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID: 3458 View all products for this Gene ID
UniProt: View information about IFN-gamma on UniProt.org

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Related Protocols

Intracellular Flow Cytometry Staining Protocol

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Other Formats

View All IFN-γ Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human IFN-γ	4S.B3	ICFC
APC anti-human IFN-γ	4S.B3	ICFC
FITC anti-human IFN-γ	4S.B3	ICFC
Biotin anti-human IFN-γ	4S.B3	ELISA Detection,ELISPOT Detection,ICFC
Purified anti-human IFN-γ	4S.B3	ELISA,WB,ICFC
Alexa Fluor® 488 anti-human IFN-γ	4S.B3	ICFC
Alexa Fluor® 647 anti-human IFN-γ	4S.B3	ICFC
Alexa Fluor® 700 anti-human IFN-γ	4S.B3	ICFC
Pacific Blue™ anti-human IFN-γ	4S.B3	ICFC
PerCP/Cyanine5.5 anti-human IFN-γ	4S.B3	ICFC
APC/Cyanine7 anti-human IFN-γ	4S.B3	ICFC
PE/Cyanine7 anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 421™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 570™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 605™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 650™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 711™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 785™ anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 510™ anti-human IFN-γ	4S.B3	ICFC
PE/Dazzle™ 594 anti-human IFN-γ	4S.B3	ICFC
APC/Fire™ 750 anti-human IFN-γ	4S.B3	ICFC
PerCP anti-human IFN-γ	4S.B3	ICFC
Brilliant Violet 750™ anti-human IFN-γ	4S.B3	ICFC
KIRAVIA Blue 520™ anti-human IFN-γ Antibody	4S.B3	ICFC
Spark NIR™ 685 anti-human IFN-γ Antibody	4S.B3	ICFC
APC anti-human IFN-γ	4S.B3	ICFC
Spark Blue™ 515 anti-human IFN-γ	4S.B3	ICFC
Spark UV™ 387 anti-human IFN-γ	4S.B3	ICFC
Spark PLUS UV395™ anti-human IFN-γ	4S.B3	ICFC
PE anti-human IFN-γ	4S.B3	ICFC
GMP APC anti-human IFN-γ	4S.B3	ICFC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 10.01.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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