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Spark PLUS UV395™ anti-mouse CD273 (B7-DC, PD-L2) Antibody

Pricing & Availability
Clone
TY25 (See other available formats)
Regulatory Status
RUO
Other Names
PD-L2, PDL2, B7DC, CD273
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
TY25_SparkPlusUV395_CD273_Antibody_1_100124
C57BL/6 mouse splenocytes were cultured overnight with recombinant mouse GM-CSF protein. Cells were preincubated with TruStain FcX™ PLUS anti-mouse CD16/32 (Cat. No. 156604) and stained with anti-mouse CD11c (clone N418) FITC and anti-mouse CD273 (B
  • TY25_SparkPlusUV395_CD273_Antibody_1_100124
    C57BL/6 mouse splenocytes were cultured overnight with recombinant mouse GM-CSF protein. Cells were preincubated with TruStain FcX™ PLUS anti-mouse CD16/32 (Cat. No. 156604) and stained with anti-mouse CD11c (clone N418) FITC and anti-mouse CD273 (B
Compare all formats See Spark PLUS UV™ 395 spectral data
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107231 25 µg 190 CHF
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107232 100 µg 390 CHF
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Description

B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. The TY25 antibody has been reported to be useful for blocking PD-1 mediated interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse B7-DC transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark PLUS UV395™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark PLUS UV395™ has a maximum excitation of 355 nm and a maximum emission of 385 nm.

Excitation Laser
Ultraviolet Laser (355 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1,6, blocking2,4,5 of PD-1 mediated interactions, and immunohistochemistry of acetone-fixed frozen sections3. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107221-107226).

Application References

(PubMed link indicates BioLegend citation)
  1. Yamazaki T, et al. 2002. J. Immunol. 169:5538. (FC, IP, WB)
  2. Ansari MJI, et al. 2003. J. Exp. Med. 198:63. (Block)
  3. Salama AD, et al. 2003. J. Exp. Med. 198:71. (IHC)
  4. Matsumoto K, et al. 2004. J. Immunol. 172:2530. (FC, Block)
  5. Yamazaki T, et al. 2005. J. Immunol. 175:1586. (Block)
  6. Meng Q, et al. 2006. Invest Opthalmol Vis Sci. 47:444. (WB) PubMed
  7. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  8. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
RRID
AB_3662165 (BioLegend Cat. No. 107231)
AB_3662165 (BioLegend Cat. No. 107232)

Antigen Details

Structure
B7 Immunoglobulin superfamily, 42 kD
Distribution

Dendritic cells, liver, few transformed cell lines, subset of macrophages; not expressed on lymphoid tissues

Function
Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor
PD-1, uncharacterized receptor
Cell Type
Dendritic cells, Macrophages
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Shin T, et al. 2003. J. Exp. Med. 198:31.
2. Liu X, et al. 2003. J. Exp. Med. 197:1721.
3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
58205 View all products for this Gene ID
UniProt
View information about CD273 on UniProt.org
Go To Top Version: 2    Revision Date: 10.01.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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