- Clone
- 5E8/CXCR2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD182, CXCR2, CD128b, IL-8RB, Type II IL-8 receptor
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CXCR2 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor B (IL-8RB), CD182, and CD128b. It is a CXC chemokine receptor belongs to G protein-coupled receptor (GPCR) family. CXCR2 is expressed as homodimer or heterodimer with CXCR1 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. CXCR2 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α,β,γ (CXCL1, CXCL2, CXCL3).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CXCR2 transfected L1.2 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: The 5E8/CXCR2 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR2 expression.
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
-
Application References
(PubMed link indicates BioLegend citation) -
- Kyriakakis E, et al. 2011. J Leukoc Biol. 90:929. PubMed.
- RRID
-
AB_3106313 (BioLegend Cat. No. 285051)
Antigen Details
- Structure
- Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR1
- Distribution
-
Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes
- Function
- Neutrophil activation, chemotaxis, angiogenesis
- Ligand/Receptor
- IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), GRO-& alpha;,ß, γ (CXCL1, CXCL2, CXCL3)
- Cell Type
- Granulocytes, NK cells, T cells, Monocytes, Endothelial cells, Oligodendrocytes, Neutrophils
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Chuntharapai A, et al. 1994. J. Immunol. 153:5682.
2. Wilson S, et al. 2005. J. Biol. Chem. 280:28663.
3. Emadi S, et al. 2005. Blood 105:464.
4. Omari KM, et al. 2005. Brain 128:1003.
5. Juremalm M and Nilsson G. 2005. Chem. Immunol. Allergy 87:130.
6. Wolf M, et al. 1998. Eur. J. Immunol. 28:164. - Gene ID
- 3579 View all products for this Gene ID
- UniProt
- View information about CD182 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
-
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD182 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human CD182 (CXCR2) | 5E8/CXCR2 | FC |
PE anti-human CD182 (CXCR2) | 5E8/CXCR2 | FC |
APC anti-human CD182 (CXCR2) | 5E8/CXCR2 | FC |
Alexa Fluor® 488 anti-human CD182 (CXCR2) | 5E8/CXCR2 | FC |
Alexa Fluor® 647 anti-human CD182 (CXCR2) | 5E8/CXCR2 | FC |
PE/Cyanine7 anti-human CD182 (CXCR2) | 5E8/CXCR2 | FC |
PerCP/Cyanine5.5 anti-human CD182 (CXCR2) | 5E8/CXCR2 | FC |
APC/Fire™ 750 anti-human CD182 (CXCR2) | 5E8/CXCR2 | FC |
PE/Dazzle™ 594 anti-human CD182 (CXCR2) | 5E8/CXCR2 | FC |
Pacific Blue™ anti-human CD182 (CXCR2) | 5E8/CXCR2 | FC |
TotalSeq™-A1273 anti-human CD182 (CXCR2) | 5E8/CXCR2 | PG |
TotalSeq™-C1273 anti-human CD182 (CXCR2) | 5E8/CXCR2 | PG |
Spark Red™ 718 anti-human CD182 (CXCR2) (Flexi-Fluor™) | 5E8/CXCR2 | FC |
TotalSeq™-B1273 anti-human CD182 (CXCR2) | 5E8/CXCR2 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD182 (CXCR2)
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PE anti-human CD182 (CXCR2)
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APC anti-human CD182 (CXCR2)
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Alexa Fluor® 488 anti-human CD182 (CXCR2)
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Alexa Fluor® 647 anti-human CD182 (CXCR2)
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PE/Cyanine7 anti-human CD182 (CXCR2)
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PerCP/Cyanine5.5 anti-human CD182 (CXCR2)
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APC/Fire™ 750 anti-human CD182 (CXCR2)
-
PE/Dazzle™ 594 anti-human CD182 (CXCR2)
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Pacific Blue™ anti-human CD182 (CXCR2)
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TotalSeq™-A1273 anti-human CD182 (CXCR2)
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TotalSeq™-C1273 anti-human CD182 (CXCR2)
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Spark Red™ 718 anti-human CD182 (CXCR2) (Flexi-Fluor™)
-
TotalSeq™-B1273 anti-human CD182 (CXCR2)
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