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Spark Red™ 718 anti-human CD81 (TAPA-1) Antibody

Pricing & Availability
Clone
5A6 (See other available formats)
Regulatory Status
RUO
Other Names
S5.7, CVID6, TSPAN28
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5A6_SparkRed718_CD81_Antibody_051624
Human peripheral blood lymphocytes were stained with anti-human CD81 (TAPA-1) (clone 5A6) Spark Red™ 718 (filled histogram). Open histogram represents unstained cells.
  • 5A6_SparkRed718_CD81_Antibody_051624
    Human peripheral blood lymphocytes were stained with anti-human CD81 (TAPA-1) (clone 5A6) Spark Red™ 718 (filled histogram). Open histogram represents unstained cells.
Compare all formats See Spark Red™ 718 spectral data
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349537 25 tests 160 CHF
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349538 100 tests 320 CHF
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Description

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1 (target of an antiproliferative antibody). CD81 is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It also has low levels of expression on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8, and CD82 in T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human OCI-LY8 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Red™ 718 has a maximum excitation of 697 nm and a maximum emission of 718 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western Blotting3 and immunoprecipitation2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265.
  2. Oren R, et al. 1990. Mol. Cell. Biol. 8:4007. (IP)
  3. Clark K, et al. 2004. J. Biol. Chem. 279(19):19401. (IP, WB)
  4. Mochida K, et al. 2008. J. Virol. 13:6711.
  5. Rappa G, et al. 2014. Mol Cancer Res. 12:1840. PubMed
RRID
AB_3662248 (BioLegend Cat. No. 349537)
AB_3662248 (BioLegend Cat. No. 349538)

Antigen Details

Structure
26 kD, type III transmembrane protein, member of the TM4SF tetraspanin family. Complexed with CD82, CD19, CD21, or CD4, CD8.
Distribution

T cells, NK, monocytes, B cells, endothelial and epithelial cells, low on granulocytes.

Function
Regulates cell activation and growth and cell aggregation.
Cell Type
B cells, Endothelial cells, Epithelial cells, Monocytes, Neural Stem Cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Transcription Factors
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265.
  2. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127.
  3. Wright M, et al. 1994. Immunol. Today 15:588.
Gene ID
975 View all products for this Gene ID
UniProt
View information about CD81 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.16.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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