- Clone
- HIB22 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V CD22.14
- Other Names
- BL-CAM, Siglec-2, Lyb8
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![HIB22_Spark-Violet-423_CD22_Antibody_071923 HIB22_Spark-Violet-423_CD22_Antibody_071923](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/HIB22_Spark-Violet-423_CD22_Antibody_071923-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human CD19 Alexa Fluor® 700 and anti-human CD22 (clone HIB22) Spark Violet™ 423 (left) or mouse IgG1, κ Spark Violet™ 423 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
302531 | 25 tests | 135 CHF | ||||
302532 | 100 tests | 300 CHF |
CD22 is a 130 kD type I transmembrane glycoprotein also known as Siglec-2 and BL-CAM. It is a member of the immunoglobulin superfamily (sialoadhesion subgroup). CD22 is expressed in the cytoplasm of pro-B and pre-B cells, and on the surface of mature B and activated B cells, but not on plasma cells. CD22 is present in the B cell receptor complex and associates with SHP-1, Syk, Lck, Lyn, and phospholipase Cγ1. A primary function of CD22 is thought to be in limiting antigen receptor signaling by modulating B cell activation threshold. CD22 has been shown to bind to CD45RO and CD75, although the natural ligands for this molecule remain controversial.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections.
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Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York.
- Clark E. 1993. J. Immunol.. 150:4715.
- Shan D and O. Press. 1995. J. Immunol.. 154:4466.
- RRID
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AB_3068223 (BioLegend Cat. No. 302531)
AB_3068223 (BioLegend Cat. No. 302532)
Antigen Details
- Structure
- Ig superfamily, a type I glycosylated integral transmembrane protein, 120-130 kD
- Distribution
-
B cells
- Function
- Adhesion, B-monocyte and B-T interactions
- Ligand/Receptor
- CD45RO, CD75
- Cell Type
- B cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules, Siglec Molecules
- Antigen References
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1. Clark E. 1993. J. Immunol. 150:4715.
2. Shan D, et al. 1995. J. Immunol. 154:4466. - Gene ID
- 933 View all products for this Gene ID
- UniProt
- View information about CD22 on UniProt.org
Other Formats
View All CD22 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human CD22 | HIB22 | FC |
PE anti-human CD22 | HIB22 | FC |
PE/Cyanine5 anti-human CD22 | HIB22 | FC |
Purified anti-human CD22 | HIB22 | FC,IHC-F |
APC anti-human CD22 | HIB22 | FC |
PE/Cyanine7 anti-human CD22 | HIB22 | FC |
PerCP/Cyanine5.5 anti-human CD22 | HIB22 | FC |
Purified anti-human CD22 (Maxpar® Ready) | HIB22 | FC,CyTOF® |
Alexa Fluor® 647 anti-human CD22 | HIB22 | FC |
APC/Fire™ 750 anti-human CD22 | HIB22 | FC |
Brilliant Violet 421™ anti-human CD22 | HIB22 | FC |
Brilliant Violet 510™ anti-human CD22 | HIB22 | FC |
APC/Cyanine7 anti-human CD22 | HIB22 | FC |
PE/Dazzle™ 594 anti-human CD22 | HIB22 | FC |
PerCP/Cyanine5.5 anti-human CD22 | HIB22 | FC |
Spark Violet™ 423 anti-human CD22 | HIB22 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD22
Human peripheral blood lymphocytes stained HIB22 FITC -
PE anti-human CD22
Human peripheral blood lymphocytes stained with HIB22 PE -
PE/Cyanine5 anti-human CD22
Human peripheral blood lymphocytes stained HIB22 PE/Cyanine5 -
Purified anti-human CD22
Human peripheral blood lymphocytes stained with purified HIB... -
APC anti-human CD22
Human peripheral blood lymphocytes stained with HIB22 APC -
PE/Cyanine7 anti-human CD22
Human peripheral blood lymphocytes were stained with CD22 (c... -
PerCP/Cyanine5.5 anti-human CD22
Human peripheral blood lymphocytes were stained with CD19 AP... -
Purified anti-human CD22 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 159Tb-an... -
Alexa Fluor® 647 anti-human CD22
Human peripheral blood lymphocytes were stained with CD22 (c... -
APC/Fire™ 750 anti-human CD22
Human peripheral blood lymphocytes were stained with CD22 (c... -
Brilliant Violet 421™ anti-human CD22
Human peripheral blood lymphocytes were stained with CD22 (c... -
Brilliant Violet 510™ anti-human CD22
Human peripheral blood lymphocytes were stained with CD22 (c... -
APC/Cyanine7 anti-human CD22
Human peripheral blood lymphocytes were stained with CD19 FI... -
PE/Dazzle™ 594 anti-human CD22
Human peripheral blood lymphocytes were stained with CD19 Br... -
PerCP/Cyanine5.5 anti-human CD22
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 423 anti-human CD22
Human peripheral blood lymphocytes were stained with anti-hu...
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