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Spark Violet™ 423 anti-human IL-2 Antibody

Pricing & Availability
Clone
W19046A (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-2, T cell growth factor (TCGF); Eosinophil differentiation factor (EDF); Killer cell helper factor (KHF); Macrophage-activating factor for cytotoxicity I (MAF-C I); Thymocyte differentiation factor (TDF)
Isotype
Rat IgG2b, κ
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Product Citations
publications
W19046A_SparkViolet423_IL2_Antibody_080423
Human peripheral blood lymphocytes were stimulated with Cell Activation Cocktail (with Brefeldin A) (Cat. No. 423303 and 423304) for 6 hours, surface stained with anti-human CD3 APC, and then treated with the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Cells were then stained with anti-human IL-2 (clone W19046A) Spark Violet™ 423 (left) or left unstained (right).
  • W19046A_SparkViolet423_IL2_Antibody_080423
    Human peripheral blood lymphocytes were stimulated with Cell Activation Cocktail (with Brefeldin A) (Cat. No. 423303 and 423304) for 6 hours, surface stained with anti-human CD3 APC, and then treated with the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Cells were then stained with anti-human IL-2 (clone W19046A) Spark Violet™ 423 (left) or left unstained (right).
Compare all formats See Spark Violet™ 423 spectral data
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310603 25 tests 160 CHF
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310604 100 tests 360 CHF
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Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human IL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.

Excitation Laser
Violet Laser (405 nm)
RRID
AB_3068183 (BioLegend Cat. No. 310603)
AB_3068183 (BioLegend Cat. No. 310604)

Antigen Details

Structure
Cytokine; 15.4 kD (Mammalian)
Distribution

T cells

Function
Upregulated by NFAT; downregulated by TCF-8 and CIF (colostrums inhibitory factor)
Ligand/Receptor
High affinity heterotrimer of IL-2Rα/β/γ, intermediate affinity homodimer IL-2Rα (CD25; p55; Tac) and heterodimer IL-2Rβ (CD122)/γ; γ-subunit (CD132) in common with IL-4R, IL-7R, IL-13R, IL-15R
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Taniguchi T, et al. 1993. Cell 73:5.
3. Nistico G. 1993. Prog. Neurobiol. 40:463.
4. Waldmann T, et al. 1993. Ann. NY Acad. Sci. 685:603.

Gene ID
3558 View all products for this Gene ID
UniProt
View information about IL-2 on UniProt.org
Go To Top Version: 1    Revision Date: 07.10.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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