Spark Violet™ 423 anti-rat CD3 Antibody, CD3, 1F4

Pricing & Availability

Clone: 1F4 (See other available formats)
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Mouse IgM, κ
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Product Citations: publications

1F4_SparkViolet423_CD3_Antibody_1_011124 — Lewis rat splenocytes were stained with anti-rat CD3 (clone 1F4) Spark Violet™ 423 (filled histogram). Open histogram represents unstained cells.

Compare all formats See Spark Violet™ 423 spectral data

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201423

25 µg

141 CHF

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201424

100 µg

352 CHF

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Description

CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: F344 rat spleen cells stimulated with PMA and calcium ionophore
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 423 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 423 has a maximum excitation of 400 nm and a maximum emission of 415 nm.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections¹ and formaldehyde- fixed paraffin embedded sections^4,5 immunofluorescence microscopy³, in vivo activation of T cell responses¹, and in vivo inhibition of T cell responses².
RRID: AB_3097534 (BioLegend Cat. No. 201423)
AB_3097534 (BioLegend Cat. No. 201424)

Antigen Details

Structure: Ig superfamily, approximately 20-25 kD
Distribution: Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC complex
Cell Type: NKT cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Tanaka T, et al. 1989 J. Immunol. 142:2791.
2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.
Gene ID: 25710 View all products for this Gene ID 300678 View all products for this Gene ID 315609 View all products for this Gene ID 25300 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD3 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-rat CD3	1F4	FC,IHC-F
FITC anti-rat CD3	1F4	FC
PE anti-rat CD3	1F4	FC
Alexa Fluor® 488 anti-rat CD3	1F4	FC,IHC-F
Alexa Fluor® 647 anti-rat CD3	1F4	FC,IHC-F
APC anti-rat CD3	1F4	FC
Ultra-LEAF™ Purified anti-rat CD3	1F4	FC,IHC-F
PerCP/Cyanine5.5 anti-rat CD3	1F4	FC
PE/Cyanine5 anti-rat CD3	1F4	FC
PE/Cyanine7 anti-rat CD3	1F4	FC
Spark Violet™ 423 anti-rat CD3	1F4	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01.11.2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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