Spark Violet™ 500 anti-human CD15 (SSEA-1) Antibody, CD15, W6D3

Pricing & Availability

Clone: W6D3 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: Lewis X, 3-FAL, 3-FL, LNFP III, Lex, SSEA-1, X-hapten
Isotype: Mouse IgG1, κ
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Product Citations: publications

W6D3_SparkViolet500_CD15_Antibody_033123 — Human peripheral blood granulocytes were stained with anti-human CD16 APC and anti-human CD15 (SSEA-1) (clone W6D3) Spark Violet™ 500 (left), or unstained control (right).

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323063

25 tests

166 CHF

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323064

100 tests

368 CHF

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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: WERI-RB-1 retinoblastoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 500 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 500 has a maximum excitation of 393 nm and a maximum emission of 500 nm.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_2936668 (BioLegend Cat. No. 323063)
AB_2936668 (BioLegend Cat. No. 323064)

Antigen Details

Structure: Poly-N-acetyllactosamine
Distribution: Neutrophils, eosinophils, monocytes
Function: Adhesion
Cell Type: Embryonic Stem Cells, Eosinophils, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Stocks SC, et al. 1990. Biochem. J. 268:275.
Gene ID: 2526 View all products for this Gene ID
UniProt: View information about CD15 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD15 Reagents Request Custom Conjugation

Description	Clone	Applications
Brilliant Violet 785™ anti-human CD15 (SSEA-1)	W6D3	FC
Purified anti-human CD15 (SSEA-1)	W6D3	FC,CyTOF®
FITC anti-human CD15 (SSEA-1)	W6D3	FC
PE anti-human CD15 (SSEA-1)	W6D3	FC
APC anti-human CD15 (SSEA-1)	W6D3	FC
Alexa Fluor® 488 anti-human CD15 (SSEA-1)	W6D3	FC
Alexa Fluor® 647 anti-human CD15 (SSEA-1)	W6D3	FC
PE/Cyanine5 anti-human CD15 (SSEA-1)	W6D3	FC
PerCP anti-human CD15 (SSEA-1)	W6D3	FC
PerCP/Cyanine5.5 anti-human CD15 (SSEA-1)	W6D3	FC
Pacific Blue™ anti-human CD15 (SSEA-1)	W6D3	FC
Alexa Fluor® 700 anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 510™ anti-human CD15 (SSEA-1)	W6D3	FC
PE/Cyanine7 anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 605™ anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 650™ anti-human CD15 (SSEA-1)	W6D3	FC
Purified anti-human CD15 (SSEA-1) (Maxpar® Ready)	W6D3	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD15 (SSEA-1)	W6D3	FC
Brilliant Violet 421™ anti-human CD15 (SSEA-1)	W6D3	FC
APC/Fire™ 750 anti-human CD15 (SSEA-1)	W6D3	FC
FITC anti-human CD15 (SSEA-1)	W6D3	FC
Pacific Blue™ anti-human CD15 (SSEA-1)	W6D3	FC
TotalSeq™-A0392 anti-human CD15 (SSEA-1)	W6D3	PG
Brilliant Violet 711™ anti-human CD15 (SSEA-1)	W6D3	FC
APC/Cyanine7 anti-human CD15 (SSEA-1)	W6D3	FC
TotalSeq™-B0392 anti-human CD15 (SSEA-1)	W6D3	PG
TotalSeq™-C0392 anti-human CD15 (SSEA-1)	W6D3	PG
PE anti-human CD15 (SSEA-1)	W6D3	FC
APC/Fire™ 810 anti-human CD15 (SSEA-1) Antibody	W6D3	FC
TotalSeq™-D0392 anti-human CD15 (SSEA-1)	W6D3	PG
GMP FITC anti-human CD15 (SSEA-1)	W6D3	FC
Spark YG™ 593 anti-human CD15 (SSEA-1)	W6D3	FC
GMP PE anti-human CD15 (SSEA-1)	W6D3	FC
GMP Pacific Blue™ anti-human CD15 (SSEA-1)	W6D3	FC
Spark Violet™ 500 anti-human CD15 (SSEA-1)	W6D3	FC
Spark UV™ 387 anti-human CD15 (SSEA-1)	W6D3	FC
PE/Cyanine7 anti-human CD15 (SSEA-1)	W6D3	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03.15.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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