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Spark Violet™ 500 anti-human CD32 Antibody

Pricing & Availability
Clone
FUN-2 (See other available formats)
Regulatory Status
RUO
Workshop
VI B051
Other Names
FCR II, FcγRII
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
FUN-2_SparkViolet500_CD32_Antibody_122022
Human peripheral blood lymphocytes were stained with anti-human CD20 APC and anti-human CD32 (clone FUN-2) Spark Violet™ 500 (left) or stained with anti-human CD20 APC only (right).
  • FUN-2_SparkViolet500_CD32_Antibody_122022
    Human peripheral blood lymphocytes were stained with anti-human CD20 APC and anti-human CD32 (clone FUN-2) Spark Violet™ 500 (left) or stained with anti-human CD20 APC only (right).
Compare all formats See Spark Violet™ 500 spectral data
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303233 25 tests 166 CHF
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303234 100 tests 368 CHF
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Description

CD32 is a 40 kD polymorphic transmembrane glycoprotein also known as FcγRII and FCRII. It is an immunoglobulin superfamily member expressed on monocytes/macrophages, granulocytes, platelets and B cells. There are at least 6 isoforms of CD32 resulting from alternative mRNA splicing. CD32 mediates phagocytosis and oxidative burst in granulocytes, as well as platelet aggregation and immunomodulation. The extracellular domain of CD32 binds to polymeric and aggregated IgG and immune complexes, while the intracellular domain has been reported to associate with SHP-1 (B1 isoform).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 500 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 500 has a maximum excitation of 393 nm and a maximum emission of 500 nm.

Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of acetone-fixed frozen tissue sections. 

Clone FUN-2 recognizes both CD32A and CD32B.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. 1997. Leucocyte Typing VI Garland Press. London.
  2. Lerino F, et al. 1993. J. Immunol. 150:1794.
  3. Personal communication.
  4. van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717.PubMed
  5. Smeltz RB. 2007. J. Immunol. 178:4786.
  6. Satta N, et al. 2011. Blood. 117:5223. PubMed.
RRID
AB_2936547 (BioLegend Cat. No. 303233)
AB_2936547 (BioLegend Cat. No. 303234)

Antigen Details

Structure
Ig superfamily, polymorphic transmembrane glycoprotein, 40 kD
Distribution

Monocytes, granulocytes, B cells, platelets

Function
Endocytosis, cytotoxicity, platelet aggregation, B cell function
Ligand/Receptor
Aggregated IgG, immune complex
Cell Type
B cells, Dendritic cells, Granulocytes, Monocytes, Platelets
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Stuart S, et al. 1989. EMBO J. 8:3657.
2. Huang Y, et al. 1999. Scand. J. Immunol. 49:177.
3. Hisaka H, et al. 1999. Pathobiology 67:92.

Gene ID
2212 View all products for this Gene ID
UniProt
View information about CD32 on UniProt.org

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

Go To Top Version: 1    Revision Date: 12.20.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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