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Spark Violet™ 538 anti-human Ig light chain κ Antibody

Pricing & Availability
Clone
TB28-2 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin light chain kappa, Ig kappa
Isotype
Mouse IgG1, κ
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Product Citations
publications
TB28-2_SPKVL-538_Ig-light-chain-kappa_Antibody_1_051823
Overnight cultured human peripheral blood lymphocytes were stained with anti-human CD19 APC and anti-human Ig light chain κ (clone TB28-2) Spark Violet™ 538 (left), or mouse IgG1, κ Spark Violet™ 538 isotype control (right).
  • TB28-2_SPKVL-538_Ig-light-chain-kappa_Antibody_1_051823
    Overnight cultured human peripheral blood lymphocytes were stained with anti-human CD19 APC and anti-human Ig light chain κ (clone TB28-2) Spark Violet™ 538 (left), or mouse IgG1, κ Spark Violet™ 538 isotype control (right).
Compare all formats See Spark Violet™ 538 spectral data
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392713 25 tests 166 CHF
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392714 100 tests 368 CHF
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Description

The TB28-2 antibody reacts with both soluble and membrane human immunoglobulin light chain kappa (κ). It does not react with human immunoglobulin light chain lambda (λ) or heavy chain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IgG κ myeloma protein CFA.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 538 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 538 has a maximum excitation of 396 nm and a maximum emission of 538 nm.

Excitation Laser
Violet Laser (405 nm)
Application Notes

The TB28-2 target antigen is also present in serum. Cell surface staining may be optimized by removing serum from the sample prior to staining.

Additional reported applications (for relevant formats) include immunohistochemical staining on frozen tissue sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Moller P, et al. 1989. Am. J. Clin. Pathol. 1:18. (IHC-F)
  2. Tierens AM, et al. 2010. Haematologica. 95:1334. (FC)
RRID
AB_3068043 (BioLegend Cat. No. 392713)
AB_3068043 (BioLegend Cat. No. 392714)

Antigen Details

Structure
Ig family
Distribution

B-Cells

Cell Type
B cells
Biology Area
Immunology
Antigen References
  1. Katzmann JA, et al. 2002. Clin Chem. 48: 1437
  2. Weinberg DS, et al. 1984. Blood. 63:1080.
  3. Johnson A, et al. 1985. Br. J. Cancer. 52: 159.
  4. Oka S, et al. 2012. J. Clin. Exp. Hematop. 52: 127.
Gene ID
3514 View all products for this Gene ID
UniProt
View information about Ig light chain kappa on UniProt.org
Go To Top Version: 1    Revision Date: 05.18.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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