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Spark Violet™ 538 anti-mouse CD45 Recombinant Antibody

Pricing & Availability
Clone
QA17A26 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5, LCA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA17A26_SPARK-VIOLET-538_CD45_Antibody_100323
C57BL/6 mouse splenocytes were stained with anti-mouse CD45 recombinant (clone QA17A26) Spark Violet™ 538 (filled histogram), or mouse IgG1, κ Spark Violet™ 538 isotype control (open histogram).
  • QA17A26_SPARK-VIOLET-538_CD45_Antibody_100323
    C57BL/6 mouse splenocytes were stained with anti-mouse CD45 recombinant (clone QA17A26) Spark Violet™ 538 (filled histogram), or mouse IgG1, κ Spark Violet™ 538 isotype control (open histogram).
Compare all formats See Spark Violet™ 538 spectral data
Cat # Size Price Quantity Check Availability Save
157621 25 µg 140 CHF
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157622 100 µg 375 CHF
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Description

CD45 is known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 538 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 538 has a maximum excitation of 396 nm and a maximum emission of 538 nm.

Excitation Laser
Violet Laser (405 nm)
Application Notes

The QA17A26 antibody can block 30-F11 clone  and vice versa. The clone QA17A26 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. Proc. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
RRID
AB_3083140 (BioLegend Cat. No. 157621)
AB_3083140 (BioLegend Cat. No. 157622)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

Expressed on almost all hematopoietic cells except for mature erythrocytes.

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Trowbridge IS and Thomas ML. 1994. Annu. Rev. Immunol. 12:85-116.
  3. Kishihara K, et al. 1993. Cell 74:143.
  4. Pulido R, et al. 1988. J. Immunol. 140:3851.

 

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org
Go To Top Version: 1    Revision Date: 10.03.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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