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Spark YG™ 570 Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Allophycocyanin, SAv-APC
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Product Citations
publications
Streptavidin_SprakYG570_2_031220
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human PNAd (clone MECA-79) Biotin, and anti-human CD8a (clone C8/144B) Alexa Fluor® 647 (green) at 4°C overnight, followed by 2.5 µg/mL of Streptavidin Spark YG™ 570 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • Streptavidin_SprakYG570_2_031220
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human PNAd (clone MECA-79) Biotin, and anti-human CD8a (clone C8/144B) Alexa Fluor® 647 (green) at 4°C overnight, followed by 2.5 µg/mL of Streptavidin Spark YG™ 570 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
  • Streptavidin_SprakYG570_3_031220
    Mouse embryonic fibroblast NIH/3T3 cell line was fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes. Then the cells were stained with anti-mouse/human CD44 biotinylated antibody (clone IM7), followed by Streptavidin Spark YG™ 570 (red). Nuclei were counterstained with DAPI (blue). This image was captured with a 40X objective.
See Spark YG™ 570 spectral data
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405281 25 µg 89 CHF
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405282 100 µg 206 CHF
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-Spark YG™ 570 is useful for detecting biotinylated antibodies. The excitation of Spark YG™ 570 by 555 nm laser light induces a light emission maximum of 570 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark YG™ 570 under optimal conditions.
Concentration
0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG™ 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.

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Go To Top Version: 1    Revision Date: 02.24.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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