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Stop Solution for TMB Substrate

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Regulatory Status
RUO
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423001 60 mL 36 CHF
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Description

Biolegend’s Stop Solution for TMB Substrate is specifically formulated to terminate the HRP/TMB reaction and stabilizing the color development for ELISA applications. TMB is a chromogenic substrate used in ELISA. TMB can act as a proton donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as HRP. The resulting diimine causes the solution to take on a blue color, and this color change can be read at 650 nm. The reaction can be stopped by addition of stop reagent, and the color turns from blue to yellow with 2-3 fold increased absorbance values at 450 nm.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • 2 x 30 mL bottles of Stop Solution

Product Details

Preparation
This solution is ready to use and requires no dilution.
Storage & Handling
Store at room temperature.
Application

ELISA
Application Notes

Each lot of Stop Solution for TMB (3,3'5,5'-tetramethylbenzidine) Substrate has been tested by ELISA. The TMB stop solution will stop TMB and HRP (horseradish peroxidase) reaction by adding the same volume as the TMB substrate added to each well. Addition of stop solution changes the color from blue to yellow. Read absorbance at 450 nm within 60 minutes. If the reader can read at 570 nm, the absorbance at 570 nm can be subtracted from the absorbance at 450 nm.

Additional Product Notes

Caution: Caustic material, strong acid. Wear eye, hand, and face protection.

Application References

(PubMed link indicates BioLegend citation)
  1. Kiianitsa K, et al. 2014. Nucleic Acids Res. 42:108. PubMed

     
Product Citations
  1. Sinani G, et al. 2017. Int J Pharm. 10.1016/j.ijpharm.2017.06.053. PubMed
  2. Maizels K 2014. Nucleic Acids Res. 42:108. PubMed
RRID
SCR_001134 (BioLegend Cat. No. 423001)

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 04.03.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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