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TotalSeq™-D1047 anti-human HLA-F Antibody

Pricing & Availability
Clone
3D11/HLA-F (See other available formats)
Regulatory Status
RUO
Other Names
HLAF, CDA12, HLA-5.4, HLA-CDA12
Isotype
Mouse IgG1, κ
Barcode Sequence
GCAACTCTCCTACCT
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373213 10 µg 369 CHF
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Description

A 42kD member of non-classical or class Ib human MHC family. It exhibits low allelic polymorphism and shorter cytoplasmic tail due to absence of exon seven. It is expressed irrespective of bound peptide. It is primarily found intracellularly on Endoplasmic Reticulum and Golgi membrane and its surface expression increases in correlation with classical MHC-I up regulation in activated monocytes, B, T, and NK cells. It is involved in inflammatory responses. It stabilizes the unstable MHC-I Open Conformers (OC) and facilitates uptake of extracellular antigen for cross presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Complex mixed with IFA, complex without adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation
The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

PG - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.


Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Additional Product Notes

TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.

Application References

(PubMed link indicates BioLegend citation)
  1. Ishitani A, et al. 2003. J. Immunol. 171:1376.
RRID
AB_3068302 (BioLegend Cat. No. 373213)

Antigen Details

Structure
42 kD Single pass type I membrane protein, heterodimer of an alpha and a Beta chain.
Distribution

B, NK, monocyte and T cells.

Function
MHC-I OC stabilization, antigen cross presentation.
Ligand/Receptor
KIR3DS1, KIR3DS4, KIR3DL2, ILT2, ILT4, TAP.
Cell Type
B cells, Monocytes, NK cells, T cells
Biology Area
Immunology
Molecular Family
MHC Antigens
Antigen References
  1. Goodridge JP, et al. 2013. J. Immunol. 191:1567.
  2. Lepin EJ, et al. 2000. Eur. J. Immunol. 30:3552.
  3. Burian A,et al. 2016. PLoS One 11:e0163297.
  4. Lee N, et al. 2010. Eur. J. Immunol. 40:2308.
Gene ID
3134 View all products for this Gene ID
UniProt
View information about HLA-F on UniProt.org
Go To Top Version: 1    Revision Date: 09.05.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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