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Ultra-LEAF™ Purified anti-human Oncostatin M Antibody

Pricing & Availability
Clone
A15044H (See other available formats)
Regulatory Status
RUO
Other Names
OSM
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A15044H_ULEAF_Oncostatin-M_Antibody_022422
Recombinant human Oncostatin M (Cat. No. 555202) (black circles) induces the proliferation of TF-1 cell. Ultra-LEAF™ purified anti-human Oncostatin M (clone A15044H) (purple squares) inhibits the effect of recombinant human Oncostatin M at 2.2 ng/mL in TF-1 cells in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype control (Cat. No. 400264) (gray triangles) does not have the effect. ND50 range: 0.5-2 μg/mL
  • A15044H_ULEAF_Oncostatin-M_Antibody_022422
    Recombinant human Oncostatin M (Cat. No. 555202) (black circles) induces the proliferation of TF-1 cell. Ultra-LEAF™ purified anti-human Oncostatin M (clone A15044H) (purple squares) inhibits the effect of recombinant human Oncostatin M at 2.2 ng/mL in TF-1 cells in a dose-dependent manner, whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype control (Cat. No. 400264) (gray triangles) does not have the effect. ND50 range: 0.5-2 μg/mL
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601654 1 mg 1205 CHF
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601653 100 µg 358 CHF
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

Human oncostatin M (OSM) was initially isolated from supernatant of U937 cells treated with PMA. It was identified by its property to inhibit the proliferation of A375 melanoma cells and other human tumor cells. Bioinformatic homology analysis reveals that OSM has a similar composition and structure to LIF, G-CSF, and IL-6. OSM and LIF are located on human chromosome 22, and additional evidence suggests that these genes were derived by duplication of a common ancestral gene. In fact, many of its biological functions are shared with LIF. OSM binds two types of OSM receptor (OSMR) complexes. The type I receptor complex is composed of gp130 (IL-6 signal transducer) and LIF receptor β (LIFRβ) subunits. The type II receptor is constituted by the gp130 receptor and the OSMR β-chain. OSM also shares the OSMRβ-chain with the IL-31 receptor complex. The OSMRβ subunit is expressed by fibroblast, endothelial, hepatic, lung, and hematopoietic cells. The proinflammatory properties of OSM have been reported in skin, adipose tissue, lung, heart, and liver tissues. OSM triggers acute phase protein synthesis in hepatocytes. OSM is a potent inhibitor of keratinocyte proliferation and decreases the expression of both early and late keratinocyte differentiation markers. OSM transcripts are elevated in skin from psoriasis and atopic dermatitis patients, as compared with healthy skin. OSM and its receptor complexes play a key role in cutaneous inflammatory responses. In addition, OSM expression is increased in colonic biopsies of patients with active inflammatory bowel disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Oncostatin M
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant human Oncostatin M (Cat. No. 555202) at 2.2 ng/mL on TF-1 cells. ND50 range: 0.5 -2 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910474 (BioLegend Cat. No. 601654)
AB_2910474 (BioLegend Cat. No. 601653)

Antigen Details

Structure
Cytokine
Distribution

Activated monocytes, macrophages, T cells including skin-infiltrating T cells, and dendritic cells

Function
Proinflammatory mediator and keratinocyte activator, induces acute phase protein synthesis, hematopoiesis, and bone remodeling
Interaction
Keratinocytes, fibroblasts, endothelia, hepatic cells, lung, hematopoietic cells, epithelial cells, and smooth muscle cells
Ligand/Receptor
Receptor complex: type I and type II complexes are formed by oncostatin M receptor (OSMR), LIFR, and p130 (IL-6 signal transducer).
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Proliferation and Viability, Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Zarling JM, et al. 1986. Proc Natl Acad Sci USA. 83:9739-43.
  2. Rose TM and Bruce AG. 1991 Proc Natl Acad Sci USA. 88: 8641-5.
  3. Mosley B, et al. 1996. J Biol Chem. 271:32635-43.
  4. Dillon SR, et al. 2004. Nat Immunol. 5:752-60.
  5. Boniface K, et al. 2007. J Immunol. 178:4615-22.
  6. Beigel F, et al. 2014. PLoS One. 9:e93498.
  7. Schnittker D, et al. 2013. Mediators Inflamm. 2013:317503.

Gene ID
5008 View all products for this Gene ID
UniProt
View information about Oncostatin M on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02.24.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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